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IP0010

Millipore

Anti-HA Immunoprecipitation Kit

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.56

antibody product type

primary antibodies

clone

monoclonal

analyte chemical class(es)

proteins (hemagglutinin)

technique(s)

immunoprecipitation (IP): suitable

storage temp.

2-8°C

General description

Anti-hemagglutinin (HA) Immunoprecipitation Kit is designed to recover maximum amounts of HA-tagged proteins in the immunoprecipitates. The process is carried out in mini-spin columns instead of microcentrifuge tubes to ensure easy washing of antigen-antibody-bound beads with minimal variability.

Application

Anti- hemagglutinin (HA) Immunoprecipitation Kit has been used for immunoprecipitation. It has also been used in in vitro acetylation assay from human embryonic kidney cell lines.

Features and Benefits

  • Anti- hemagglutinin (HA) coupled beads enable high specific binding to HA-tagged fusion proteins
  • Coupling of Anti-HA antibodies to the beads eliminates other preliminary and calibration steps
  • Mini-spin columns enable the minimal loss of anti-HA-bound beads during washing
  • The CelLyticTM M cell lysis reagent provides a high yield, rapid and efficient mammalian cell lysis
  • Ready-to-use cell lysis reagent and 10X concentrated immunoprecipitation buffer

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Kit Components Also Available Separately

Product No.
Description
SDS

  • A2095Monoclonal Anti-HA−Agarose antibody produced in mouse, clone HA-7, purified immunoglobulin, PBS suspension .5 mLSDS

  • C2978CelLytic M, Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization. 50 mLSDS

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

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Dietary interventions such as intermittent fasting (IF) have emerged as an attractive strategy for cancer therapies; therefore, understanding the underlying molecular mechanisms is pivotal. Here, we find SIRT7 decline markedly attenuates the anti-tumor effect of IF. Mechanistically, AMP-activated protein kinase
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Connexin-43 (Cx43, also known as GJA1) is the most ubiquitously expressed connexin isoform in mammalian tissues. It forms intercellular gap junction (GJ) channels, enabling adjacent cells to communicate both electrically and metabolically. Cx43 is a short-lived protein which can be

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