I6137

Monoclonal Anti-Bovine IgM antibody produced in mouse

clone BM-23, ascites fluid

• UNSPSC Code: 12352203
• NACRES: NA.46
• MDL number: MFCD00164546
• Technique(s): indirect ELISA: 1:4,000, western blot: suitable, capture ELISA: suitable, immunoprecipitation (IP): suitable
• Conjugate: unconjugated
• Application: ELISA (c), ELISA (i), IP, WB
• Citations: 8

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: unconjugated
• antibody form: ascites fluid
• antibody product type: secondary antibodies
• clone: BM-23, monoclonal
• contains: 15 mM sodium azide
• technique(s): indirect ELISA: 1:4,000, western blot: suitable, capture ELISA: suitable, immunoprecipitation (IP): suitable
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Bovine IgMs are glycoprotein antibodies that regulate antigen binding during immunological responses. These antibodies have three isotypes, namely, IgMa, IgMb and IgMc. Structural restraints on movement enhance the complement-fixing functions of bovine IgMs . Monoclonal Anti-Bovine IgM antibody detects an epitope on the heavy chain of bovine IgM. The antibody reacts with bovine serum, but does not react with sera from human, baboon, marmoset, gibbon, rhesus, hamster, rabbit, goat, pig, dog, rat, turkey, chicken, or catfish. Furthermore, the product does not bind to native or reduced bovine light chains.

Monoclonal Anti-Bovine IgM (mouse IgG1 isotype) is derived from the hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse. IgM is highly conserved antibody in vertebrates and is expressed early during immune response. It exists as pentamer and is secreted by peritoneal B cells.

Immunogen

Purified bovine IgM

Application

Monoclonal Anti-Bovine IgM antibody is suitable for use in indirect ELISA (1:1,000), western blot, immunoprecipitation and ELISA.

Biochem/physiol Actions

IgM plays a key role in engulfing of the of apoptotic cells. A reduction in serum IgM levels leads to increased autoimmune response and higher risk for infections. IgM displays polyreactive and autoreactive functionality. It plays a key role in tissue homeostasis by mediating clearance of tissue based molecules.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable