FLE250
Firefly Lantern Extract
for ATP determination
Synonym(s):
FLE
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1 VIAL
$144.00
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1 VIAL
$144.00
About This Item
UNSPSC Code:
12352202
NACRES:
NA.81
$144.00
Check Cart for Availability
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form
solution
packaging
pkg of 250 mg dried lanterns
technique(s)
toxicology assay: suitable
storage temp.
−20°C
Related Categories
General description
The bioluminescent organism, Photinus pyralis firefly is the source of firefly lantern extract.[1] The luciferase enzyme is 60 kDa enzyme which lacks prosthetic group and bound metal in its structure.[2] The N-terminal harbors residues crucial for the bioluminescent activity and the C-terminal possess peroxisome targeting motif.[1]
Application
Fireflies have been used in several studies of bioluminescence. In particular they may be used in assays to determine toxicity pathways because bioluminescence can quantitatively measure a wide range of biological activities.
Biochem/physiol Actions
Firefly Lantern Extract containing luciferase catalyzes oxidative decarboxylation of luciferin (LH2) in the presence of ATP, oxygen and Mg2+ to yield a light-emitter oxyluciferin.[2]
Other Notes
Crude luciferin and luciferase
Reconstitution
After reconstitution with 25 mL of water, vial will contain 0.06 M potassium phosphate, pH 7.4, and 0.02 M MgSO4.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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