FD40S
Fluorescein isothiocyanate–dextran
average mol wt 40,000
Synonym(s):
FITC–Dextran
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About This Item
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biological source
synthetic (organic)
conjugate
FITC conjugate
form
powder
mol wt
average mol wt 40,000
extent of labeling
0.003-0.020 mol FITC per mol glucose
color
yellow to dark orange
solubility
H2O: soluble 25 mg/mL, clear to slightly hazy, yellow to deep orange
storage temp.
2-8°C
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General description
Dextran is a polymer of anhydroglucose. It is composed of approximately 95% α-D-(166) linkages. The remaining (163) linkages account for the branching of dextran. Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million. FITC-dextran has an excitation maximum of 490 nm. It has a pH range of 5 to 9.
Application
Dextran labeled with fluorescein isothiocyanate for possible use in perfusion studies in animals.
Fluorescein isothiocyanate–dextran (FITC-dextran, fluorescein-dextran, FITC) was used in the following processes:
- Fluorescein-dextran microscopy.
- To evaluate the in vivo pulmonary vascular permeability in polymorphonuclear leukocyte α-defensins expressing transgenic mice.
- To quantify endocytic activity by flow cytometry.
- To label α-lactalbumin and ribonuclease.
Linkage
Similar to FD-40, but produced by Sigma.
Preparation Note
Sigma typically tests the solubility of FITC dextrans in water at concentrations at or above 25 mg/ml. Solutions should be protected from light. In vivo, FITC-dextran is stable for more than 24 hours.
Other Notes
Native dextran has been found to have a molecular weight (MW) in the range of 9 million to 500 million. Lower MW dextrans will exhibit slightly less branching and have a more narrow range of MW distribution. Dextrans with MW greater than 10,000 behave as if they are highly branched. As the MW increases, dextran molecules attain greater symmetry. Dextrans with MW of 2,000 to 10,000, exhibit the properties of an expandable coil. At MW below 2,000, dextran is more rod-like. The MW of dextran is measured by one or more of the following methods: low angle laser light scattering, size exclusion chromatography, copper-complexation and anthrone reagent colorometric reducing-end sugar determination and viscosity.
To gain a comprehensive understanding of our extensive range of Dextrans for your research, we encourage you to visit our Carbohydrates Category page.
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Warning
hcodes
Hazard Classifications
Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
target_organs
Respiratory system
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
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Related Content
Dextran is a polymer of anhydroglucose. It is composed of approximately 95% alpha-D-(166) linkages. The remaining (163) linkages account for the branching of dextran.
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