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F5803

Sigma-Aldrich

o-Dianisidine dihydrochloride

Suitable for use in glucose determination

Synonym(s):

3,3′-Dimethoxybenzidine dihydrochloride, Fast Blue B

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About This Item

Empirical Formula (Hill Notation):
C14H16N2O2 · 2HCl
CAS Number:
20325-40-0
Molecular Weight:
317.21
Beilstein/REAXYS Number:
3917996
EC Number:
243-737-5
MDL number:
MFCD00012488
UNSPSC Code:
12171500
PubChem Substance ID:
24894894
NACRES:
NA.47

form

crystalline

pH range

6.95-7.05

mp

268 °C

solubility

water: 2.5 mg/mL, colorless

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-8°C

SMILES string

Cl.Cl.COc1cc(ccc1N)-c2ccc(N)c(OC)c2

InChI

1S/C14H16N2O2.2ClH/c1-17-13-7-9(3-5-11(13)15)10-4-6-12(16)14(8-10)18-2;;/h3-8H,15-16H2,1-2H3;2*1H

InChI key

UXTIAFYTYOEQHV-UHFFFAOYSA-N

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General description

A preweighed vial containing 50 mg o-Dianisidine.

Specificity

o-Dianisidine (3,3′-dimethoxybenzidine) is a peroxidase substrate suitable for use in ELISA procedures. This substrate produces a soluble end product that is yellow-orange in color and can be read spectrophotometrically at 405 nm. The reaction may be stopped with 5 M HCl.

Application

o-Dianisidine dihydrochloride is suitable for use in the glucose determination. It is suitable reagent used in the following studies:
  • As substrate for the determination of serum ceruloplasmin from its oxidase activity.
  • Determination of total antioxidant response (TAR) against potent free radical reactions by a novel, colorimetric and fully automated method.
  • As colorimetric indicator for the simultaneous semi-quantitative estimation of glucose, lactate and uric acid on paper-based microfluidic devices (microPAD).
  • Myeloperoxidase activity assay.

Warning

Probably carcinogenic.

enzyme

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Description
Pricing

standard

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signalword

Danger

Hazard Classifications

Acute Tox. 4 Oral - Carc. 1B - Eye Dam. 1 - Skin Corr. 1

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type P3 (EN 143) respirator cartridges

Certificates of Analysis (COA)

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Wijitar Dungchai et al.
Analytica chimica acta, 674(2), 227-233 (2010-08-04)
We report here the use of multiple indicators for a single analyte for paper-based microfluidic devices (microPAD) in an effort to improve the ability to visually discriminate between analyte concentrations. In existing microPADs, a single dye system is used for
Measurement of ceruloplasmin from its oxidase activity in serum by use of o-dianisidine dihydrochloride.
K H Schosinsky et al.
Clinical chemistry, 20(12), 1556-1563 (1974-12-01)
G B Penner et al.
Journal of dairy science, 92(4), 1725-1733 (2009-03-25)
This study was conducted to determine the effects of feeding Fermenten (Church and Dwight Co., Princeton, NJ) with or without dietary sucrose on ruminal fermentation, apparent total-tract nutrient digestibility, and nutrient utilization. Eight ruminally cannulated Holstein cows (163 +/- 55
L O Chow et al.
Journal of dairy science, 91(4), 1534-1543 (2008-03-20)
Objectives of the study were to evaluate the effect of planting date on in vitro neutral detergent fiber digestibility (IVFD) of whole-crop barley (Hordeum vulgare) and its effects on productivity of lactating dairy cows. Two cultivars of barley were planted
G B Penner et al.
Journal of dairy science, 92(6), 2767-2781 (2009-05-19)
The objective of the study was to investigate the fractional rate of volatile fatty acid (VFA) absorption and the expression of genes encoding for transporters and enzymes involved in the absorption and metabolism of VFA in ruminal tissue when cattle
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Protocols

Enzymatic Assay of Diamine Oxidase (E.C. No. 1.4.3.22)

To standardize a procedure for the enzymatic assay of Diamine Oxidase.

Enzymatic Assay: Glucose Oxidase

Glucose oxidase activity measured via continuous spectrophotometric assay at 500 nm, indicating glucose oxidation rate.

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