F4051

Proteasome Fraction II from rabbit

5 mg/mL, Reticulocyte cell extract, solution

• UNSPSC Code: 12352200
• NACRES: NA.77
• MDL number: MFCD03458197
• Form: solution

Properties

• Quality Segment: 200
• form: solution
• concentration: 5 mg/mL
• storage temp.: −70°C

Description

General description

Manufactured for Sigma by Boston Biochem., Inc.

Application

Proteasome Fraction II is ideal for demonstrating ubiquitin- and ATP-dependent degradation/conjugation of radiolabeled or immunodetectable substrates. Isopeptidase inhibitor and a proteasome inhibitor are highly recommended for the accumulation of ubiquitin protein conjugates. Typical assay concentration for FII is 0.5 - 4 mg/ml.

Biochem/physiol Actions

Essentially ubiquitin and ATP free. Contains E1, most E2s, UCHs, and the proteasome 20S and 26S isoforms.

Protein fraction of a cell extract that binds to anion-exchange resin.

Physical form

Solution in 25 mM HEPES, pH 7.6.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable