D9779
DL-Dithiothreitol
for molecular biology, ≥98% (HPLC), ≥99% (titration)
Synonym(s):
(±)-Dithiothreitol, rac-Dithiothreitol, Dithiothreitol, threo-1,4-Dimercapto-2,3-butanediol, Cleland’s reagent, DTT
About This Item
Recommended Products
grade
for molecular biology
assay
≥98% (HPLC)
≥99% (titration)
form
powder
reaction suitability
reagent type: reductant
mp
41-44 °C (lit.)
solubility
H2O: 50 mg/mL
cation traces
heavy metals (as Pb): ≤5 ppm
suitability
suitable for molecular biology
foreign activity
DNase, RNase, protease, none detected
storage temp.
2-8°C
SMILES string
O[C@H](CS)[C@H](O)CS
InChI
1S/C4H10O2S2/c5-3(1-7)4(6)2-8/h3-8H,1-2H2/t3-,4-/m1/s1
InChI key
VHJLVAABSRFDPM-QWWZWVQMSA-N
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General description
Application
It has been used:
- as a component for protein extraction in western blot analysis
- to prepare sample lysis buffer for quantitative mass spectroscopy
- as a kinase buffer component for enzyme-linked immunosorbent assay (ELISA)
Biochem/physiol Actions
Features and Benefits
- Suitable for molecular biology
- RNase, DNase, Exonuclease, and Protease-free
- High purity (HPLC ≥98%)
- No heavy metal ≤5ppm
Other Notes
Comparable product
signalword
Danger
hcodes
Hazard Classifications
Acute Tox. 4 Oral - Eye Dam. 1 - Skin Irrit. 2
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
Certificates of Analysis (COA)
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Protocols
Follow this DDT reduction protocol to reduce disulfide bonds in thiol-modified oligonucleotides.
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