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D7876

Diamine Oxidase from porcine kidney

≥0.05 unit/mg solid

Synonym(s):

Amine:oxygen oxidoreductase (deaminating) (pyridoxal-containing)

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250 mg

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$99.40
1 g

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$319.00
5 g

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$969.00
10 g

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About This Item

CAS Number:
9001-53-0
EC Number:
1.4.3.22 (BRENDA, IUBMB)
UNSPSC Code:
12352204
EC Number:
232-613-6
NACRES:
NA.54
MDL number:
MFCD00130946
Specific activity:
≥0.05 unit/mg solid
Biological source:
Porcine kidney

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biological source

Porcine kidney

Quality Segment

200

form

solid

specific activity

≥0.05 unit/mg solid

mol wt

170 kDa

solubility

100 mM sodium phosphate buffer, pH 7.2: soluble 10 mg/mL

foreign activity

monoamine oxidase (benzylamine substrate) ≤1%

storage temp.

−20°C

General description

Diamine oxidase from porcine kidney is a homodimer consisting of two equal subunits with a molecular weight of 87 kDa each. Each subunit contains one molecule of pyridoxal phosphate and one atom of copper. The molecular mass of the enzyme is found to be 170 kDa.[1] The enzyme is a glycoprotein containing 5% hexose, 3.3% glucosamine, 2.6% N-acetylglucosamine, and 0.25% N-acetylneuraminic acid. The enzyme exhibits a high affinity for concanavalin A.[2] Optimum pH with cadverine and histamine as substrates is found to be 6.3-7.4.

Application

Diamine oxidase from porcine kidney has been used in a study to investigate a luminescence-based test for determining ornithine decarboxylase activity. Diamine oxidase from porcine kidney has also been used in a study to investigate N-linked oligosaccharide structures in diamine oxidase.
Diamine Oxidase from porcine kidney has been used in the construction of histamine biosensor.

Biochem/physiol Actions

Diamine Oxidase catalyzes the oxidation of monoamines, diamines, and histamine to aldehydes, ammonia, and hydrogen peroxide. The enzyme is classified as a copper amine oxidase and it is a key enzyme in nitrogen metabolism. Diamine oxidase is inhibited by diethyldithiocarbamate, phenylhydrazine, semicarbazide, cyanide, isonicotinic acid hydrazide.

Other Notes

One unit will oxidize 1.0 μmole of putrescine per hr at pH 7.2 at 37 °C.

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Diamine Oxidase from porcine kidney ≥0.05 unit/mg solid

Sigma-Aldrich

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Sigma-Aldrich

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Sigma-Aldrich

A2580

Angiotensin Converting Enzyme from porcine kidney

Leucine Aminopeptidase, microsomal from porcine kidney Type VI-S, lyophilized powder, ≥12 units/mg protein (biuret)

Sigma-Aldrich

L6007

Leucine Aminopeptidase, microsomal from porcine kidney

specific activity

≥0.05 unit/mg solid

specific activity

≥1.5 units/mg solid

specific activity

≥10 units/mg protein (Bradford)

specific activity

≥12 units/mg protein (biuret)

biological source

Porcine kidney

biological source

-

biological source

-

biological source

Porcine kidney

form

solid

form

powder

form

lyophilized powder

form

lyophilized powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

solubility

100 mM sodium phosphate buffer, pH 7.2: soluble 10 mg/mL

solubility

-

solubility

-

solubility

-

mol wt

170 kDa

mol wt

-

mol wt

-

mol wt

320 kDa

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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