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D5688

Sigma-Aldrich

Dilution Solution

Extract-N-Amp Plant Dilution Solution

Synonym(s):

Direct PCR, Direct PCR dilution solution for plants, Extract‑N‑Amp Dilution Solution

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.55

form

liquid

storage temp.

−20°C

General description

Dilution Solution is intended for use with Sigma′s Extract-N-Amp Plant PCR Kit. Once the DNA is extracted from the leaf tissue, the Dilution Solution is added to the extract to neutralize inhibitory substances. The extract is then ready for PCR.

Application

Dilution Solution (Extract-N-Amp™ Plant Dilution Solution) has been used in direct PCR amplification and to prepare template DNA.

Legal Information

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

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XNAPE

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R4775

REDExtract-N-Amp™ PCR ReadyMix™, Ready-to-use 2X PCR Master Mix with Loading Dye

E3004

Extract-N-Amp™ PCR ReadyMix™, Amplifications to support Extract-N-Amp Plant and Extract-N-Amp Tissue

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Extract-N-Amp™ PCR Diluent, colorless, storage temp.:2-8°C

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E0781

Extract-N-Amp™ PCR ReadyMix™ with qPCR Reference dye, 125 mL sufficient for 10000 amplifications, hotstart, dNTPs included

pictograms

Exclamation markEnvironment
GHS07,GHS09

signalword

Warning

Hazard Classifications

Aquatic Chronic 2 - Eye Irrit. 2 - Skin Irrit. 2 - Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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Jose L Ramirez et al.
Journal of insect science (Online), 8, 1-7 (2008-04-01)
Homalodisca vitripennis (Germar) (Hemiptera: Cicadellidae), the glassy-winged sharpshooter, is one of the most important vectors of the bacterium, Xylella fastidiosa subsp. piercei (Xanthomonadales: Xanthomonadaceae) that causes Pierce's Disease in grapevines in California. In the present study we report a new
Anneke van der Zee et al.
BMC infectious diseases, 14, 27-27 (2014-01-16)
Resistance to carbapenem antibiotics is emerging worldwide among Enterobacteriaceae. To prevent hospital transmission due to unnoticed carriage of carbapenemase producing micro-organisms in newly admitted patients, or follow-up of patients in an outbreak setting, a molecular screening method was developed for
Molecular Diagnosis of Urinary Tract Infections by Semi-Quantitative Detection of Uropathogens in a Routine Clinical Hospital Setting.
van der Zee A
PLoS ONE, 11(3), e0150755-e0150755 (2016)
Daniela Hegebarth et al.
Plant, cell & environment, 40(9), 1761-1776 (2017-05-10)
The aliphatic waxes sealing plant surfaces against environmental stress are generated by fatty acid elongase complexes, each containing a β-ketoacyl-CoA synthase (KCS) enzyme that catalyses a crucial condensation forming a new C─C bond to extend the carbon backbone. The relatively
Anneke van der Zee et al.
BMC research notes, 4, 150-150 (2011-05-28)
To facilitate automation, a novel DNA extraction method for MRSA was adopted. The MRSA specific chromosome-SCCmec PCR was adapted, additional primers were added, and the performance was validated. From various laboratories in The Netherlands we received a total of 86
View All Related Papers

Protocols

REDExtract-N-Amp™ Plant PCR Kit Protocol

REDExtract-N-Amp Plant PCR kit includes loading dye for easy gel loading after plant genomic DNA extraction.

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