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D2532

Sigma-Aldrich

Denhardt′s Solution 50x

for Northern and Southern blotting, solution

Synonym(s):

blocking reagent, blocking solution, denhardts, hybridization buffer, hybridization solution, northern blocking solution, pre-hybridization, southern blocking solution

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About This Item

UNSPSC Code:
12161700
NACRES:
NA.25

Quality Level

300

form

solution

technique(s)

Southern blotting: suitable

foreign activity

DNAses, RNases, NICKases, endonucleases, and exonucleases, none detected

storage temp.

−20°C

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General description

Denhardt′s Solution is a blocking agent routinely used to reduce non-specific binding of detection reagents to the Northern and Southern Blot membranes. This 50x concentrate can be diluted to any concentration for use in a variety of pre-hybridization and hybridization buffers.

Components

Denhardt′s Solution is 1% Bovine Serum Albumin (BSA), 1 % Ficoll, and 1 % Polyvinylpyrrolidone (PVP) in water.

related product

Product No.
Description
Pricing

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Galanin receptor 3--a potential target for acute pancreatitis therapy.
Barreto SG
Neurogastroenterology and Motility, 23(3), 23(3)-23(3) (2011)
J Stokes et al.
Investigative ophthalmology & visual science, 41(7), 1629-1638 (2000-06-14)
The administration of glucocorticoids as topical or systemic medications may lead to the development of ocular hypertension through the induction of morphologic and biochemical changes in the trabecular meshwork leading to a reduction in the facility of aqueous outflow. Glucocorticoids
E J Mackie et al.
Journal of cell science, 103 ( Pt 3), 765-771 (1992-11-01)
The extracellular matrix glycoprotein, tenascin, is associated in vivo with mesenchyme undergoing osteogenesis and chondrogenesis, but is absent from mature bone and cartilage matrix. The expression of tenascin by osteoblastic cells in vitro has been investigated by immunoblotting and immunocytochemistry.
Gregor Obernosterer et al.
Nature protocols, 2(6), 1508-1514 (2007-06-16)
Here we describe a method for sensitive and specific histological detection of microRNAs (miRNAs) by in situ hybridization. The protocol focuses on the use of locked nucleic acids (LNAs), which are bi-cyclic RNA analogs that allow a significant increase in
Kazuya Morikawa et al.
PLoS pathogens, 8(11), e1003003-e1003003 (2012-11-08)
It has long been a question whether Staphylococcus aureus, a major human pathogen, is able to develop natural competence for transformation by DNA. We previously showed that a novel staphylococcal secondary sigma factor, SigH, was a likely key component for
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Northern & Southern Blot Protocols

Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.

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