CS0005-1KT Cholesterol Quantification Assay kit is suitable for use with serum and heparinized plasma samples.
Anticoagulants such as oxalate, citrate or EDTA yield slightly lower total cholesterol values.
CS0005
Cholesterol Quantification Assay kit
Synonym(s):
Cholesterol Assay kit, cholesteryl ester, free cholesterol, total cholesterol
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| Size/SKU | Availability | Price |
|---|---|---|
1 kit | Check Cart for Availability | $597.00 |
About This Item
NACRES:
NA.83
UNSPSC Code:
12352202
$597.00
Check Cart for Availability
usage
sufficient for 100 or 400 colorimetric or fluorometric tests
shipped in
dry ice
storage temp.
−20°C
General description
Cholesterol is a lipid that constitutes 20-25% of the structural components of cell membranes. Cholesterol presence assure membrane structural fluidity. Cholesterol also plays a role as an intermediate in several hormones and bile acid biosynthesis and vitamin D. Cholesterol also regulates the functions of the transporters and signaling proteins present on the plasma membrane. The major sites of cholesterol synthesis are the small intestine and liver. Cholesterol circulates in the blood as both the free acid and as cholesterol esters. Increased cholesterol levels are associated with the development of atherosclerosis and cardiovascular diseases. Thus, serum cholesterol regulation has an important therapeutic role.
The Cholesterol Quantification Assay Kit provides a simple and quick procedure for detecting and measuring very low concentrations of Cholesterol. This kit can measure free cholesterol, cholesteryl esters, or total cholesterol (which constitutes both free cholesterol and cholesteryl esters). The cholesterol concentration is determined via a coupled enzymatic reaction. This kit allows for either fluorometric or colorimetric detection, to allow maximum flexibility and a large detection range. The linear ranges are as follows:
The Cholesterol Quantification Assay Kit provides a simple and quick procedure for detecting and measuring very low concentrations of Cholesterol. This kit can measure free cholesterol, cholesteryl esters, or total cholesterol (which constitutes both free cholesterol and cholesteryl esters). The cholesterol concentration is determined via a coupled enzymatic reaction. This kit allows for either fluorometric or colorimetric detection, to allow maximum flexibility and a large detection range. The linear ranges are as follows:
- Fluorescence assay: 0.1–0.5 μg
- Colorimetric assay: 1–5 μg
Application
- Measuring free cholesterol, cholesteryl esters, or total cholesterol in biological samples (For example: Serum and Plasma and tissue culture).
- Measurement can be done either by fluorometric or colorimetric detection.
Suitable for the detection of free cholesterol, cholesteryl esters, or total cholesterol in biological samples.
Features and Benefits
- Highly sensitive, simple and high-throughput assay for measuring free cholesterol, cholesteryl esters, or total cholesterol in biological samples
- User friendly: No need to weigh or calculate needed amounts of reagents on your own
- Supportive calculator (Click here to download a calculator excel file): Will calculate the reagents needed according to your experimental needs, and analyses your results based on your experimental data!
- Quick instruction bench card - to assure your experimental success
- Suitable for both colorimetric and fluorometric detection
- More experiments in one kit - contains sufficient reagents for 100 colorimetric or 400 fluorometric tests.
- Detection time: only 30 minutes
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This Item | |||
|---|---|---|---|
| description - | description Supplied by Roar Biomedical, Inc. | description Cholesterol Oxidase, Streptomyces sp. CAS 9028-76-6, is a native cholesterol oxidase that catalyzes the oxidation of cholesterol to cholesterone and hydrogen peroxide. | description lyophilized powder, ≥20 units/mg protein |
| usage sufficient for 100 or 400 colorimetric or fluorometric tests | usage sufficient for 100 fluorometric tests | usage - | usage - |
| shipped in dry ice | shipped in - | shipped in ambient | shipped in dry ice |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. −20°C | storage temp. −20°C |
hcodes
pcodes
Hazard Classifications
Aquatic Chronic 3
Storage Class
10 - Combustible liquids
wgk
WGK 3
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Is it suitable for both seruma and plasma samples? Is it suitable for plasma collected in EDTA tubes?
1 answer-
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When do the actual serum samples that you want to analyze added. I see in Table 1, those are the standards. Table 2 lists the reagents for the reaction mix. Does that mix with table 1? This is for a biochemistry lab and we would like to analyze serums.
1 answer-
The samples (including serum) are added as aseprate set of well and are not mixed with the Table 1 standards.
Each well receives 50 µL of sample/standard/blank first, followed by addition of the reaction mix prepared using the reagents listed in Table 2. There will be wells for samples, standards, and a standard blank.
Below see a brief summary:1. Prepare standard wells and sample wells.
2. Add the appropriate amount of reaction mix.
3. Measure the absorbance (assuming colorimetric detection)Preparation
Page 2 covers sample preparation first, followed by standard preparation.
Sample Preparation :
Determine an appropriate sample amount. The final volume should be 50 µL.
For example, assuming 50 µL is the correct sample volume, dispense 50 µL of the sample into a single well of a 96-well plate.
1. Standard Preparation:
Perform an 8-fold dilution of the 2 µg/µL stock to reach 0.25 µg/µL to prepare a 1x standard.
Follow the instructions to prepare 12 standard wells, with a final volume of 50 µL each, using that diluted stock.
2. Prepare the reaction mix:
Determine the amount of reaction mix to prepare. Determine if total or free cholesterol is to be measured.
Table 2 shows how to prepare the reaction mix in a single vial and which wells to add to.
If measuring 13 wells for total cholesterol, multiply each component (assay buffer, probe, enzyme mix, cholesterol esterase) by 13.
3. Begin the assay reaction
Add 50 µL of the prepared reaction mix to each sample and standard well.
Proceed with the remaining steps described in the technical bulletin provided at the link below.https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/product/documents/129/825/cs0005bul-ms.pdf
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You are advising to use black plates for fluorescence assays. Are you talking about black plates with black bottom or black plates with optical bottom (see-through) ?
1 answer-
Black plates with clear, flat bottoms are recommended for bottom reading instruments, while solid black plates are recommended for top reading instruments.
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How can I determine the shelf life / expiration / retest date of this product?
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If this product has an expiration or retest date, it will be shown on the Certificate of Analysis (COA, CofA). If there is no retest or expiration date listed on the product's COA, we do not have suitable stability data to determine a shelf life. For these products, the only date on the COA will be the release date; a retest, expiration, or use-by-date will not be displayed.
For all products, we recommend handling per defined conditions as printed in our product literature and website product descriptions. We recommend that products should be routinely inspected by customers to ensure they perform as expected.
For products without retest or expiration dates, our standard warranty of 1 year from the date of shipment is applicable.
For more information, please refer to the Product Dating Information document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/418/501/product-dating-information-06-25-mk.pdfHelpful?
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How is shipping temperature determined? And how is it related to the product storage temperature?
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Products may be shipped at a different temperature than the recommended long-term storage temperature. If the product quality is sensitive to short-term exposure to conditions other than the recommended long-term storage, it will be shipped on wet or dry-ice. If the product quality is NOT affected by short-term exposure to conditions other than the recommended long-term storage, it will be shipped at ambient temperature. As shipping routes are configured for minimum transit times, shipping at ambient temperature helps control shipping costs for our customers. For more information, please refer to the Storage and Transport Conditions document: https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/316/622/storage-transport-conditions-mk.pdf
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