CLLS1127
DLD1 Cells TP53 -/-
human male colon (Source Disease: Colorectal adenocarcinoma)
Sign In to View Organizational & Contract Pricing.
Select a Size
Change View
| Size/SKU | Availability | Price |
|---|---|---|
1 set | Check Cart for Availability | $5,030.00 |
About This Item
UNSPSC Code:
41106514
NACRES:
NA.81
Biological source:
human male colon (Source Disease: Colorectal adenocarcinoma)
Product Name
DLD1 Cells TP53 -/-,
biological source
human male colon (Source Disease: Colorectal adenocarcinoma)
Quality Segment
OMIM accession no.
storage temp.
−196°C
Gene Information
human ... TP53(7157)
General description
DLD1 Cells TP53-/- are colorectal adenocarcinoma, epithelial cells, from a human adult male, with a ZFN double knock out modification.
This product corresponds to ATCC Cat. No. CCL-221.
This product corresponds to ATCC Cat. No. CCL-221.
Application
Media Renewal changes two to three times per week.
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.
Subculture Ratio: approx. 1.3-1.10
The base medium for this cell line is RPMI, Catalog No. R5886. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Catalog No. F4135, to a final concentration (v/v) of 10%, L-glutamine, Catalog No. G7513, to a final concentration of 2 mM and sodium pyruvate, Catalog No. S8636, to a final concentration of 1 mM.
The cell freezing medium is DMSO 1X (Catalog Number C6164).
Rapidly thaw vial by gentle agitation in 37°C water bath (~2 minutes), keeping vial cap out of the water. Decontaminate with 70% ethanol, add 9 mL culture media and centrifuge 125 x g (5-7 minutes). Resuspend in complete culture media and incubate at 37°C in a 5% CO2 atmosphere.
Subculture Ratio: approx. 1.3-1.10
The base medium for this cell line is RPMI, Catalog No. R5886. To make the complete growth medium, add the following components to the base medium: fetal bovine serum, Catalog No. F4135, to a final concentration (v/v) of 10%, L-glutamine, Catalog No. G7513, to a final concentration of 2 mM and sodium pyruvate, Catalog No. S8636, to a final concentration of 1 mM.
The cell freezing medium is DMSO 1X (Catalog Number C6164).
Features and Benefits
Zinc finger nuclease (ZFN) double knock out on chromosome 17p13.1
The DLD-1 cells are adherent, with a doubling time of approx. 20 hours.
The DLD-1 cells are adherent, with a doubling time of approx. 20 hours.
Analysis Note
Tested for Mycoplasma, sterility, post-freeze viability, short terminal repeat (STR) analysis for cell line identification, and PCR assay for cell line species confirmation.
1 of 1
This Item | |||
|---|---|---|---|
| description - | description - | description NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA. | description from human(normal adult), multiple organ |
| biological source human male colon (Source Disease: Colorectal adenocarcinoma) | biological source human male colon (Source Disease: Colorectal adenocarcinoma) | biological source human esophagus | biological source human (normal adult) |
| storage temp. −196°C | storage temp. −196°C | storage temp. - | storage temp. - |
| Quality Level 200 | Quality Level 200 | Quality Level 100 | Quality Level 100 |
| OMIM accession no. | OMIM accession no. | OMIM accession no. - | OMIM accession no. - |
| Gene Information human ... TP53(7157) | Gene Information human ... AKT2(208) | Gene Information - | Gene Information - |
Choose from one of the most recent versions:
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.