C8114

Anti-α-E-Catenin antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• UNSPSC Code: 12352203
• NACRES: NA.41
• MDL number: MFCD05665180
• Conjugate: unconjugated
• Clone: polyclonal
• Application: ARR, ICC, WB
• Citations: 12

Properties

• biological source: rabbit
• conjugate: unconjugated
• antibody form: IgG fraction of antiserum
• antibody product type: primary antibodies
• clone: polyclonal
• form: buffered aqueous solution
• mol wt: antigen 102 kDa
• species reactivity: rat, human, canine
• technique(s): immunocytochemistry: 1:200 using methanol-fixed, dog MDCK and human MCF7 cellc, microarray: suitable, western blot: 1:1,000 using whole cell extract of human epidermal carcinoma A431 cell line and cytosolic fraction of rat embryonic brain.
• UniProt accession no.: P35221
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... CTNNA1(1495) ; rat ... Ctnna1(307505)

Description

General description

α-E-Catenin is a predominant subtype of α-catenin. It is widely expressed but at low levels in the nervous system. Alternative spliced variants of α-E-Catenin include α1- and α2-E-catenin.

The catenins (α, β, γ) are cytoplasmic proteins found in varying abundance in many developing and adult tissues.

Immunogen

synthetic peptide corresponding to a region located near the C-terminus of human α-E-catenin (amino acids 873-887). This sequence is identical in mouse and Xenopus α-E-catenin. It is not found in α-N-catenin, β-catenin, and γ-catenin.

Application

Anti-α-E-Catenin antibody produced in rabbit has been used in western blotting and immunocytochemistry.

Biochem/physiol Actions

Catenins bind directly or indirectly to the conserved cytoplasmic tail domain of the cell adhesion adherins. The association of catenins to cadherins produces a complex, which is linked to the actin filament network. Catenins/cadherin complexes play an important role in mediating cell adhesion, transduction of cell-cell contact positional signals to the cell interior, and may play a crucial role in cell differentiation. The linkage of the epithelial cadherin /uvomorulin to actin is essential for the cell binding function of this cadherin. α-Catenin (CAP102, 102 kDa), originally described as an E-cadherin associated protein, has been shown to associate with other members of the cadherin family members, N-cadherin and P-cadherin. Within its conserved region α-catenin shows 30% identity to vinculin.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)