C7749 does not have an affinity tag.
C7749
Carbonic Anhydrase II bovine
≥90% (SDS-PAGE), recombinant, expressed in E. coli, ≥5,000 units/mg protein, buffered aqueous solution
Synonym(s):
Bovine carbonic anhydrase, Carbonic anhydrase enzyme
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| Size/SKU | Availability | Price |
|---|---|---|
500 μg | Check Cart for Availability | $313.00 |
5 mg | Check Cart for Availability | $676.00 |
recombinant
expressed in E. coli
Quality Segment
assay
≥90% (SDS-PAGE)
form
buffered aqueous solution
specific activity
≥5,000 units/mg protein
mol wt
~29 kDa
shipped in
wet ice
storage temp.
−20°C
Application
Carbonic anhydrase is used to create carbon dioxide capture systems and to research various purification techniques . Carbonic anhydrase is also used to study acid-base regulation in fish and carbonic anhydrase type II deficiency syndrome . Product C7749, Bovine carbonic anhydrase II (CA II), has been widely used as a model protein in the investigation of the protein folding process.
Carbonic Anhydrase II bovine has been used as a control in native gel electrophoresis and X-ray absorption spectroscopy characterization of Thalassiosira weissflogii carbonic anhydrase 1.
Biochem/physiol Actions
Carbonic Anhydrase is a zinc-containing enzyme that catalyzes the reversible conversion of carbon dioxide to bicarbonate. One of its main physiological roles is to maintain the acid-base balance in blood and other tissues. Lack of carbonic anhydrase results in carbonic anhydrase type II deficiency syndrome, which is an autosomal recessive disease that causes osteopetrosis, renal tubular acidosis and brain calcifications . Carbonic anhydrases are metalloenzymes since most active sites contain zinc. Carbonic anhydrases are widely distributed in plant and animal tissues where they are involved in diverse physiological processes, such as photosynthesis, pH homeostasis, calcification, and bone resorption. The enzymes can be localized in the cytosol or mitochondria, be membrane bound with extracellular domains, or be secreted. Bovine carbonic anhydrase II (CA II) is a cytosolic, single-chain, approximately 29 kDa enzyme.
Physical form
Supplied as a solution in 20 mM Tris, pH 7.6, with 150 mM NaCl.
Other Notes
One unit will decrease th pH of a 20 mM tris buffer from pH 8.3 to 6.3 per minute at 0 °C.
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This Item | |||
|---|---|---|---|
| description ≥90% (SDS-PAGE), recombinant, expressed in E. coli, ≥5,000 units/mg protein, buffered aqueous solution | description recombinant, expressed in E. coli, buffered aqueous solution | description lyophilized powder, ≥3,000 W-A units/mg protein | description lyophilized powder, ≥2,000 W-A units/mg protein |
| assay ≥90% (SDS-PAGE) | assay - | assay - | assay - |
| specific activity ≥5,000 units/mg protein | specific activity ≥5,000 units/mg protein | specific activity ≥2475 units/mg solid, ≥3,000 W-A units/mg protein | specific activity ≥2,000 W-A units/mg protein |
| recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant - | recombinant - |
| form buffered aqueous solution | form buffered aqueous solution | form lyophilized powder | form lyophilized powder |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| mol wt ~29 kDa | mol wt small subunit 10-12 kDa, large subunit 17-22 kDa | mol wt 30 kDa | mol wt - |
Storage Class
10 - Combustible liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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