C6607

Caspase 10 human

>90% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, 8,000 units/mg protein (Bradford)

• Synonym(s): Flice2, Mch4
• UNSPSC Code: 12352200
• NACRES: NA.32
• MDL number: MFCD03456342
• Specific activity: 8,000 units/mg protein (Bradford)
• Assay: >90% (SDS-PAGE)
• Recombinant: expressed in E. coli

Properties

• recombinant: expressed in E. coli
• Quality Segment: 200
• assay: >90% (SDS-PAGE)
• form: lyophilized powder
• specific activity: 8,000 units/mg protein (Bradford)
• mol wt: ~30 kDa
• solubility: PBS: soluble
• UniProt accession no.: Q92851
• shipped in: dry ice
• storage temp.: −70°C
• Gene Information: human ... CASP10(843)

Description

Biochem/physiol Actions

Caspase 10 has two death effector domains (DEDs) that bind to the DED in the adapter molecule FADD and recruits both TNFR1 and CD95 to form complexes with these receptors. Caspase 10 cleaves and activates caspases 3, 4, 6, 7, 8 and 9 which causes the proteolytic cleavage of many key proteins such as PARP. Based on gene expression studies, caspase 10 may be crucial in embryonic development. In view of its structural homology to caspase 8, the initiator caspase in death receptor-mediated apoptosis, caspase 10 may function in a similar and redundant manner.

Physical form

Lyophilized powder containing 0.052% ammonium sulfate, 0.158% Tris−HCl, and 0.76% sodium chloride.

Other Notes

One unit will hydrolyze 1 nmol of the caspase substrate IETD-pNA to IETD and p-nitroaniline per hour at pH 7.2 at 37 °C.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable