C6098
Monoclonal Anti-CCR-6 antibody produced in mouse
clone 53103.111, purified immunoglobulin, lyophilized powder
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About This Item
MDL number:
UNSPSC Code:
51111800
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
53103.111, monoclonal
form
lyophilized powder
species reactivity
human
technique(s)
flow cytometry: suitable using human PBMCs (1 μg/106 cells)
immunohistochemistry: 25 μg/mL using frozen human tissue sections
isotype
IgG2b
UniProt accession no.
storage temp.
−20°C
Gene Information
human ... CCR6(1235)
mouse ... Ccr6(12458)
General description
Chemokine receptor CCR6 plays an important role in adaptive immunity, recruitment of CD4+ T cells and dendritic cells to sites of epithelial inflammation. It is expressed on leukocyte subsets of T cells, B cells, immature dendritic cells, neutrophils, monocytes and NKT cells. CCR6 binds the chemokine, CCL20 (macrophage inflammatory protein-3α) and induces proinflammatory signals such as TNF-α, IFN-γ and toll-like receptor agonists that in turn activate ERK1/2 and p38 MAPK pathways. CCR6 has been linked to autoimmune diseases of multiple sclerosis, rheumatoid arthritis, psoriasis and atherosclerosis. Monoclonal Anti-CCR-6 reacts with CCR-6 transfected cells and not with the parent cell line. The antibody shows no cross-reactivity with CCR-1, CCR-2, CCR-3, CCR-4, CCR-5, CCR-7, CXCR-1, CXCR-2, CXCR-3, CXCR-4 and STRL33 transfected cells.
Specificity
The antibody reacts with human CCR-6 transfectant cells and not the parent cell line. The antibody shows no cross-reactivity with CCR-1, CCR-2, CCR-3, CCR-4, CCR-5, CCR-7, CXCR-1, CXCR-2, CXCR-3, CXCR-4 or STRL33.
Immunogen
human CCR-6 transfected NSO mouse myeloma cells.
Application
Monoclonal Anti-CCR-6 antibody may be used for flow cytometry at a working concentration of 1μg/106 cells using human peripheral blood mononuclear cells. Antibody concentration of 25 μg/ml may be used for immunohistochemistry of frozen human tissue sections.
Physical form
Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.
Preparation Note
Purified using protein G.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
11 - Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Toshihiro Ito et al.
Experimental cell research, 317(5), 613-619 (2011-03-08)
Chemokines are key mediators of leukocyte recruitment during pathogenic insult and also play a prominent role in homeostasis. While most chemokine receptors bind to multiple chemokines, CCR6 is unique in that this receptor is one of only a few that
Iain Comerford et al.
BioEssays : news and reviews in molecular, cellular and developmental biology, 32(12), 1067-1076 (2010-10-19)
Chemokines (chemotactic cytokines) drive and direct leukocyte traffic. New evidence suggests that the unusual CCR6/CCL20 chemokine receptor/ligand axis provides key homing signals for recently identified cells of the adaptive immune system, recruiting both pro-inflammatory and suppressive T cell subsets. Thus
Wuzhou Wan et al.
Trends in cardiovascular medicine, 21(5), 140-144 (2012-06-27)
Atherosclerosis is a complex vascular pathology characterized in part by accumulation of innate and adaptive inflammatory cells in arterial plaque. Molecular mediators responsible for inflammatory cell accumulation in plaque include specific members of the chemokine family of leukocyte chemoattractants and
Evemie Schutyser et al.
Cytokine & growth factor reviews, 14(5), 409-426 (2003-09-02)
CCL20, alternatively named liver and activation-regulated chemokine (LARC), macrophage inflammatory protein-3alpha (MIP-3alpha) or Exodus-1, is the only chemokine known to interact with CC chemokine receptor 6 (CCR6), a property shared with the antimicrobial beta-defensins. The ligand-receptor pair CCL20-CCR6 is responsible
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