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C2488

Sigma-Aldrich

Citrate Buffer Solution, 0.09 M

Synonym(s):

Citric Acid Buffer Solution

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About This Item

UNSPSC Code:
12161700
eCl@ss:
32129211
NACRES:
NA.25

description

Contains 1% chloroform
pH 4.8 at 25 °C.

Quality Level

form

solution

concentration

0.09 M

pH

4.8 (25 °C)

application(s)

diagnostic assay manufacturing

storage temp.

2-8°C

Related Categories

General description

Citrate Buffer Solution, 0.09 M, is a high-quality biochemical buffer solution commonly employed in various research applications. Its applications include use in acid phosphatase reactions alongside p-nitrophenyl phosphate (pNPP) enzyme substrate. Citrate Buffer is also used for the isolation of RNA from cells and tissues. In addition, it is also used as a background electrolyte for separating sulfonamides utilizing capillary zone electrophoresis. With its superior buffering capacity and ability to maintain a stable pH, Citrate Buffer Solution, 0.09 M is an excellent choice for any researcher in need of reliable performance.

Application

Citrate Buffer Solution, 0.09 M has been used as a heat-induced antigen retriever on formalin-fixed paraffin-embedded (FFPE) tissue sections during immunohistochemistry (IHC) experiments. It has also been used in the preparation of hybridization solutions for fluorescence in situ hybridization (FISH) procedures.

Other Notes

Unit definition: One unit of acid phosphatase will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per, minute at pH 4.8 at 37 °C.

pictograms

Health hazard

signalword

Warning

hcodes

Hazard Classifications

Carc. 2 - STOT RE 2 Oral

target_organs

Liver,Kidney

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter


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Migration behavior and separation of sulfonamides in capillary zone electrophoresis. I. Influence of buffer pH and electrolyte modifier
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Journal of Chromatography A, 755(2), 261-269 (1996)
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