C1622
Cyclohexanone Monooxygenase from Acinetobacter sp.
≥12 U/mL, ammonium sulfate suspension, recombinant, expressed in E. coli
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| Size/SKU | Availability | Price |
|---|---|---|
1 mL | Check Cart for Availability | $146.00 |
About This Item
CAS Number:
UNSPSC Code:
12352204
NACRES:
NA.54
EC Number:
MDL number:
Recombinant:
expressed in E. coli
Concentration:
≥12 U/mL
$146.00
Check Cart for Availability
recombinant
expressed in E. coli
Quality Segment
form
ammonium sulfate suspension
concentration
≥12 U/mL
storage temp.
−20°C
Application
Cyclohexanone monooxygenase has been used in a study that cloned and overexpressed the 2-oxo-Δ(3)-4,5,5-trimethylcyclopentenylacetyl-CoA monooxygenase (OTEMO) in Escherichia coli. Cyclohexanone monooxygenase has also been used in a study that investigated the effects of structural modification of the cell wall on the biotransformation capability by recombinant Corynebacterium glutamicum cells.
Biochem/physiol Actions
Purified cyclohexanone monooxygenase has a molecular weight of 59,000 Da. It is a versatile oxygenation catalyst. The enzyme uses the bound FAD-4a-OOH oxygenating intermediate to initiate transfer of oxygen to electrophilic substrate sites. The reaction consequently yields the corresponding sulfoxide and selenoxide products. This enzyme is also capable of oxygenating at nitrogen, trivalent phosphorus, and boron sites in boronic acids. Hence, it is one of the most broad-based flavoprotein oxygenases known.[1]
Physical form
Suspension in 80% saturated ammonium sulfate, 20 mM K-Na-phosphate buffer pH 7, 3.5 mM 1,4-Dithioerythritol (DTE)
Other Notes
1 unit corresponds to the amount of enzyme which catalyzes the cyclohexanone-stimulated oxidation of 1 μmol of NADPH per minute at pH 9.0 and 30 °C
1 of 1
This Item | |||
|---|---|---|---|
| form ammonium sulfate suspension | form ammonium sulfate suspension | form ammonium sulfate suspension | form ammonium sulfate suspension |
| recombinant expressed in E. coli | recombinant expressed in E. coli | recombinant - | recombinant expressed in E. coli |
| concentration ≥12 U/mL | concentration - | concentration 300-600 units/mL | concentration - |
| storage temp. −20°C | storage temp. 2-8°C | storage temp. 2-8°C | storage temp. 2-8°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
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