B3275

Monoclonal Anti-Rabbit Immunoglobulins–Biotin antibody produced in mouse

clone RG-16, purified immunoglobulin, buffered aqueous solution

• Synonym(s): Monoclonal Anti-Rabbit Immunoglobulins (IgG, IgA, IgM)
• NACRES: NA.46
• UNSPSC Code: 12352203
• Conjugate: biotin conjugate
• Clone: RG-16, monoclonal
• Application: ELISA (d), IHC (p), WB
• Citations: 22

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: biotin conjugate
• antibody form: purified immunoglobulin
• antibody product type: secondary antibodies
• clone: RG-16, monoclonal
• form: buffered aqueous solution
• species reactivity: rabbit
• should not react with: human, pig, chicken, bovine, horse, guinea pig, turkey, rat, canine, goat, feline, sheep
• technique(s): direct ELISA: 1:60,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,500, western blot: 1:200,000-1:400,000 using using an assay detecting actin in total cell extract of HeLa cells (5-10 μg per well), western blot: 1:200,000
• isotype: IgG1
• shipped in: dry ice
• storage temp.: −20°C
• target post-translational modification: unmodified

Description

General description

Reacts with an epitope on the heavy chain of rabbit IgG, IgA and IgM, which is sensitive to reduction. No cross-reactivity with human IgG, IgA and IgM.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

The mouse monoclonal anti-Rabbit IgG (heavy chain-specific) peroxidase conjugated antibody can be used as a secondary antibody for western blot analysis of protein preps from E. coli cultures at a concentration of 1:10000 diluted in PBSt for 1 hour at room temperature.

Biochem/physiol Actions

Binds only rabbit Igs. Does not react with reduced rabbit Igs.

IgG antibody subtype is the most abundant of serum immunoglobulins of the immune system. It is secreted by B cells and is found in blood and extracellular fluids and provides protection from infections caused by bacteria, fungi and viruses. Maternal IgG is transferred to fetus through the placenta that is vital for immune defense of the neonate against infections. IgA antibody is secretory antibody and is present abundantly in mucous linings of gastrointestinal, respiratory and genitourinary tracts, tears and saliva. IgM antibodies are present as pentamers in the serum and are produced in response to antigens.

Physical form

Solution in 0.01 M phosphate buffered saline pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable