A9655

Auramine O

Dye content, ≥80%, certified by the Biological Stain Commission, powder

• Synonym(s): 4,4′-(Imidocarbonyl)bis(N,N-dimethylaniline) monohydrochloride, Basic Yellow 2, Pyoctaninum aureum
• Empirical Formula (Hill Notation): C₁₇H₂₁N₃ · HCl
• CAS Number: 2465-27-2
• Molecular Weight: 303.83
• UNSPSC Code: 12171500
• NACRES: NA.47
• PubChem Substance ID: 24891484
• EC Number: 219-567-2
• MDL number: MFCD00012484
• Colour Index Number: 41000
• Beilstein/REAXYS Number: 4030061

Properties

• Product Name: Auramine O, Dye content ≥80 %, certified by the BSC
• agency: certified by the BSC
• Quality Level: 200
• form: powder
• composition: Dye content, ≥80%
• technique(s): microbe id | staining: suitable
• color: yellow to green
• mp: >250 °C (dec.) (lit.)
• solubility: ethanol: 1 mg/mL, yellow to orange
• λmax: 370 nm in H₂O, 432 nm in H₂O

Description

General description

Certified for use by fluorescence microscopy in Churukian′s modification of Truant′s fluorescent method for acid fast bacilli on paraffin sections.

Auramine O is used for the staining of acid-fast organisms like coccidia. Along with carbol, it generates brilliant yellow fluorochrome of tubercle bacilli. It is effective in detecting positive cases of tuberculosis. Auramine O binds to the mycolic acid in the bacterial cell wall.

Application

Auramine O has been used to study its absorption by a natural biopolymer poly (γ-glutamic acid).

Safety Information

• pictograms: GHS08,GHS07,GHS09
• signalword: Warning
• hcodes: H302,H319,H351,H411
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2
• wgk: WGK 3
• pcodes: P202 - P264 - P273 - P301 + P312 - P305 + P351 + P338 - P308 + P313
• Storage Class: 11 - Combustible Solids