A7549
Anti-Apoptosis-Inducing Factor antibody produced in rabbit
IgG fraction of antiserum, buffered aqueous solution
Synonym(s):
Anti-AIF
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About This Item
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biological source
rabbit
conjugate
unconjugated
antibody form
IgG fraction of antiserum
antibody product type
primary antibodies
clone
polyclonal
form
buffered aqueous solution
mol wt
antigen 57 kDa
species reactivity
mouse, human
packaging
antibody small pack of 25 μL
technique(s)
microarray: suitable
western blot: 1:1,000 using human epitheloid carcinoma HeLa cell extract and mouse brain extract
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Gene Information
human ... AIFM1(9131)
mouse ... Aifm1(26926)
General description
Apoptosis-Inducing Factor (AIF) is a mitochondrial flavoprotein that can induce apoptosis in isolated nuclei. Studies have reported that AIF can induce the realease of cytochrome c and caspase 9. Bcl-2 is known to inhibit AIF release without affecting its apoptotic functions.
Rabbit Anti-AIF antibody recognizes human and mouse AIF (57kDa). Staining of AIF in immunoblotting is specifically inhibited with the AIF immunizing peptide (human, amino acids 593-613).
Rabbit Anti-AIF antibody recognizes human and mouse AIF (57kDa). Staining of AIF in immunoblotting is specifically inhibited with the AIF immunizing peptide (human, amino acids 593-613).
Apoptosis-inducing factor (AIF) is encoded by the gene mapped to human chromosome X (Xq25-Xq26). It is a 57 kDa mitochondrial flavoprotein. AIF contains two mitochondrial localization sequences and two putative nuclear localization sequences.
Immunogen
synthetic peptide corresponding to the C-terminus of human Apoptosis Inducing Factor (AIF), (amino acids 593-613), conjugated to KLH.
Application
Protein lysates from 2CLL, 10 MCl and Jeko-1 cells were analyzed by western blot using anti-AIF as the primary antibody. Cells also underwent intracellular delivery by being transfected with anti-AIF at a final dilution of 1:2500 and then analyzed by FLOW cytometry.
Rabbit Anti-Apoptosis-Inducing Factor (AIF) antibody has been used for immunoblot analysis at 1:1000 and 1:2000 dilutions. The antibody can also be used for microarray applications.
Biochem/physiol Actions
Microinjection of apoptosis-inducing factor (AIF) into the cytoplasm of intact cells induces chromatin condensation, dissipation of the mitochondrial transmembrane potential and exposure of phosphatidylserine in the plasma membrane.
Physical form
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Life with or without AIF
Hangen E, et al.
Trends in Biochemical Sciences, 35(5), 278-287 (2010)
Export of mitochondrial AIF in response to proapoptotic stimuli depends on processing at the intermembrane space
Otera H, et al.
The Embo Journal, 24(7), 1375-1386 (2005)
Ricardo Parrondo et al.
Molecular cancer, 9, 182-182 (2010-07-14)
NF-kappaB is a transcription factor that promotes inhibition of apoptosis and resistance to chemotherapy. It is commonly believed that inhibition of NF-kappaB activity can increase sensitivity of cancer cells to chemotherapy. However, there is evidence that NF-kappaB activation can sensitize
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