A7345

Anti-V5 Agarose Affinity Gel antibody produced in mouse

purified immunoglobulin, clone V5-10

• Synonym(s): Monoclonal Anti-V5
• UNSPSC Code: 12352203
• NACRES: NA.56
• MDL number: MFCD03457579
• Conjugate: agarose conjugate
• Clone: V5-10, monoclonal
• Application: IP, affinity chromatography
• Citations: 161

Properties

• biological source: mouse
• Quality Level: 200
• conjugate: agarose conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: V5-10, monoclonal
• analyte chemical class(es): proteins (V5)
• technique(s): affinity chromatography: suitable, immunoprecipitation (IP): suitable
• capacity: 2.5 nmol/mL, gel (V5 fusion protein)
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Anti-V5 Agarose Affinity Gel is made of purified immunoglobulin fraction of monoclonal anti-V5 isolated from ascites fluid of the V5-10 hybridoma coupled to cyanogen bromide-activated beaded agarose. The purified monoclonal antibody is immobilized on agarose.

The Anti-V5 Agarose Affinity Gel is composed of the monolonal anti-V5 antibody conjugated to cyanogen bromide-activated beaded agarose. The antibody recognizes the V5-tagged recombinant fusion proteins expressed in transfected mammalian cells.

Application

Anti-V5 Agarose Affinity Gel antibody produced in mouse has been used in immunoprecipitation[1][2][3] and recombinant protein purification.[4][5]

Biochem/physiol Actions

Recognizes V5 Tag (GKPIPNPLLGLDST) fusion proteins

Recombinant DNA technology enables the attachment of genes of interest to specific sequences or genes that can provide ′affinity handles′ designed to enable the selective identification and purification of the protein of interest.[6] The addition of a V5 tag sequence (GKPIPNPLLGLDST)[6] to a given gene creates a stable fusion product that does not appear to interfere with the bioactivity of the protein, or with the biodistribution of the tagged product. Many recombinant proteins have been engineered to express a short sequence derived from the V5 from simian virus known as V5-tag.[7] Anti-“tag” agarose conjugates may facilitate the detection, isolation and purification of these proteins.[7]

Physical form

Suspension of beaded agarose (1:1 v/v) in 0.01 M phosphate buffered saline, containing 15 mM sodium azide.

Preparation Note

For continuous use and extended storage, store at 2 °C to 8 °C. Do not freeze.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable