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A5062

Anti-Guinea Pig IgG (whole molecule)−Alkaline Phosphatase antibody produced in goat

affinity isolated antibody, buffered aqueous glycerol solution

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0.25 mL
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$107.00
0.5 mL
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$187.00
1 mL
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$301.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.46
MDL number:
MFCD00162365
Conjugate:
alkaline phosphatase conjugate
Clone:
polyclonal
Application:
ELISA (d), IHC (p), WB
Citations:
12

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biological source

goat

Quality Level

200

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous glycerol solution

technique(s)

direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000

shipped in

wet ice

storage temp.

2-8°C

target post-translational modification

unmodified

General description

Immunoglobulin G (IgG) is a glycoprotein antibody that regulates immune responses such as phagocytosis and is also involved in the development of autoimmune diseases . In guinea pig IgG is subdivided into-IgG1 and IgG2. IgG2 can bind to Fc receptor of macrophages and monocytes. Anti-guinea pig IgG (whole molecule) −alkaline phosphatase antibody can be used in immunohistology (diluted 1:50). Goat anti-guinea pig IgG (whole molecule) −alkaline phosphatase antibody reacts specifically with guinea pig IgG.

Immunogen

Purified guinea pig IgG.

Application

Anti-guinea pig IgG (whole molecule) −alkaline phosphatase antibody can be used in direct ELISA (diluted 1:30,000) and dot blot.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)
Western blot analysis of SF9 cells was performed using alkaline phosphatase conjugated goat anti-guinea pig IgGat 1:10000 as the secondary.

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1 mM MgCl2, 10 mM glycine, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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This Item
A3187A3312A3438
Anti-Guinea Pig IgG (whole molecule)−Alkaline Phosphatase antibody produced in goat affinity isolated antibody, buffered aqueous glycerol solution

Sigma-Aldrich

A5062

Anti-Guinea Pig IgG (whole molecule)−Alkaline Phosphatase antibody produced in goat

Anti-Human IgG (γ-chain specific)−Alkaline Phosphatase antibody produced in goat affinity isolated antibody, buffered aqueous glycerol solution

Sigma-Aldrich

A3187

Anti-Human IgG (γ-chain specific)−Alkaline Phosphatase antibody produced in goat

Anti-Human IgG (γ-chain specific), F(ab′)2 fragment−Alkaline Phosphatase antibody produced in goat affinity isolated antibody, buffered aqueous glycerol solution

Sigma-Aldrich

A3312

Anti-Human IgG (γ-chain specific), F(ab′)2 fragment−Alkaline Phosphatase antibody produced in goat

Anti-Mouse IgG (γ-chain specific)−Alkaline Phosphatase antibody produced in goat affinity isolated antibody, buffered aqueous glycerol solution

Sigma-Aldrich

A3438

Anti-Mouse IgG (γ-chain specific)−Alkaline Phosphatase antibody produced in goat

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

conjugate

alkaline phosphatase conjugate

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

biological source

goat

biological source

goat

biological source

goat

biological source

goat

technique(s)

direct ELISA: 1:30,000, western blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50

technique(s)

direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000

technique(s)

direct ELISA: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000

technique(s)

direct ELISA: 1:30,000, dot blot: 1:30,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50, western blot: 1:30,000

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

form

buffered aqueous glycerol solution

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificates of Analysis (COA)

Lot/Batch Number
SLBW1968
SLBQ2508V
SLBJ0713V
SLBD3248V
SLBC2189V

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Related Content

Data Sheet - A5062

Instructions

Philippe P Pagni et al.
Diabetes, 63(6), 2015-2025 (2014-02-13)
Type 1 diabetes is thought to be an autoimmune condition in which self-reactive T cells attack insulin-secreting pancreatic β-cells. As a proinflammatory cytokine produced by β-cells or macrophages, interleukin-1β (IL-1β) represents a potential therapeutic target in diabetes. We reasoned IL-1β
Anahita Daryabeigi et al.
Genetics, 203(2), 733-748 (2016-04-22)
SUN (Sad1 and UNC-84) and KASH (Klarsicht, ANC-1, and Syne homology) proteins are constituents of the inner and outer nuclear membranes. They interact in the perinuclear space via C-terminal SUN-KASH domains to form the linker of nucleoskeleton and cytoskeleton (LINC)
M D Alexander et al.
Immunology, 35(1), 115-123 (1978-07-01)
Guinea-pig IgG2 and IgT1 bind to contiguous Fc receptors on homologous peritoneal macrophages. Equilibrium association constants determined for the binding of human IgG subclasses to homologous peripheral blood monocytes show that the order of binding is IgG1 greater than IgG3
View All Related Papers

Global Trade Item Number

SKUGTIN
A5062-.25ML04061837514395
A5062-.5ML04061837514401
A5062-1ML04061833375419

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