A4487

Aphidicolin

Ready Made Solution, from Nigrospora sphaerica

• Synonym(s): ICI 69653, NSC-234714
• Empirical Formula (Hill Notation): C₂₀H₃₄O₄
• CAS Number: 38966-21-1
• Molecular Weight: 338.48
• UNSPSC Code: 51102829
• NACRES: NA.85
• EC Number: 200-664-3
• MDL number: MFCD00083214

Properties

• biological source: Nigrospora sphaerica
• Quality Level: 200
• vapor pressure: 0.55 hPa ( 20 °C)
• form: liquid, ready-to-use solution
• color: clear colorless
• solubility: H₂O: miscible (completely)
• antibiotic activity spectrum: neoplastics, viruses
• mode of action: DNA synthesis | interferes, enzyme | inhibits
• storage temp.: −20°C
• SMILES string: O[C@]1([C@H]2C[C@@]3([C@@]4([C@H]([C@@]([C@@H](CC4)O)(CO)C)CC[C@H]3C2)C)CC1)CO
• InChI: 1S/C20H34O4/c1-17(11-21)15-4-3-13-9-14-10-19(13,7-8-20(14,24)12-22)18(15,2)6-5-16(17)23/h13-16,21-24H,3-12H2,1-2H3/t13-,14+,15-,16+,17-,18-,19-,20-/m0/s1
• InChI key: NOFOAYPPHIUXJR-APNQCZIXSA-N

Description

General description

Aphidicolin is a tetracyclic diterpene with antiviral and antimitotic properties used for cell cycle synchronization in various cell lines.[1]

Application

Aphidicolin, from Nigrospora sphaerica, was used for cell cycle synchronization of tobacco BY-2 cells. It is used to block the entry of cells into S-phase.[2]

Biochem/physiol Actions

Aphidicolin specifically inhibits DNA polymerase α that is responsible for DNA replication. It also inhibits α-like DNA polymerases of plants and yeasts but does not inhibit synthesis of RNA and proteins. Aphidicolin competes for the dCTP-specific binding site on DNA polymerase α specifically. Aphidicolin has antiviral and antimitotic properties. It blocks the cell cycle at S phase. It has been shown to induce apoptosis in HeLa cells.[3]

Packaging

1 ml

Physical form

Aphidicolin Ready Made Solution supplied as a 1 mg/ml solution in DMSO.

Other Notes

Keep container tightly closed in a dry and well-ventilated place. Storage class (TRGS 510): Combustible liquids

Safety Information

• Storage Class: 10 - Combustible liquids
• flash_point_f: 188.6 °F - closed cup
• flash_point_c: 87 °C - closed cup