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A3449

Acrylamide/Bis-acrylamide solution

30%, BioReagent, suitable for electrophoresis, 19:1

Synonym(s):

30% Acrylamide/Bisacrylamide 19:1, Polyacrylamide solution

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$84.75

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$231.00

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About This Item

UNSPSC Code:
41105319
PubChem Substance ID:
329770824
NACRES:
NA.25
MDL number:
MFCD00080848

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Product Name

Acrylamide/Bis-acrylamide, 30% solution, BioReagent, suitable for electrophoresis, 19:1

sterility

0.2 μm filtered

Quality Level

200

product line

BioReagent

form

liquid

feed ratio

19:1

technique(s)

electrophoresis: suitable

suitability

suitable for electrophoresis

foreign activity

Protease, none detected

storage temp.

2-8°C

SMILES string

NC(=O)C=C.C=CC(=O)NCNC(=O)C=C

InChI

1S/C7H10N2O2.C3H5NO/c1-3-6(10)8-5-9-7(11)4-2;1-2-3(4)5/h3-4H,1-2,5H2,(H,8,10)(H,9,11);2H,1H2,(H2,4,5)

InChI key

OCQZXMCGTAWGEQ-UHFFFAOYSA-N

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Acrylamide/Bis-acrylamide, 30% solution BioReagent, suitable for electrophoresis, 19:1

Sigma-Aldrich

A3449

Acrylamide/Bis-acrylamide, 30% solution

Acrylamide/Bis-acrylamide, 30% solution BioReagent, suitable for electrophoresis, 29:1

Sigma-Aldrich

A3574

Acrylamide/Bis-acrylamide, 30% solution

Acrylamide/Bis-acrylamide, 40% solution BioReagent, suitable for electrophoresis, 19:1

Sigma-Aldrich

A9926

Acrylamide/Bis-acrylamide, 40% solution

Acrylamide/Bis-acrylamide BioReagent, 19:1 (ratio), Molecular Biology

Sigma-Aldrich

A2917

Acrylamide/Bis-acrylamide

form

liquid

form

liquid

form

liquid

form

powder

suitability

suitable for electrophoresis

suitability

suitable for electrophoresis

suitability

suitable for electrophoresis

suitability

-

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

electrophoresis: suitable

technique(s)

-

feed ratio

19:1

feed ratio

29:1

feed ratio

19:1

feed ratio

19:1 (ratio)

foreign activity

Protease, none detected

foreign activity

Protease, none detected

foreign activity

-

foreign activity

DNAse (exonuclease), NICKase (endonuclease), RNAse, and protease, none detected

sterility

0.2 μm filtered

sterility

0.2 μm filtered

sterility

0.2 μm filtered

sterility

-

General description

Polyacrylamide gels are prepared using acrylamide and bis-acrylamide, where bis-acrylamide is a cross-linking agent. On the other hand, acrylamide is a water soluble monomer which helps in forming a transparent stable insoluble gel.

Application

These 30% (w/w) acrylamide/bis-acrylamide solutions are available at the most common ratios for use in protein and nucleic acid electrophoresis. The concentration is based on the total weight of both the acrylamide and bis-acrylamide.
Acrylamide/Bis-acrylamide, 30% solution has been used as a constituent in the preparation of 5% stacking gel and 7% resolving gel for sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).

Preparation Note

Solution prepared from electrophoresis grade acrylamide and bis-acrylamide in ultra-pure water.

pictograms

Health hazardExclamation mark
GHS08,GHS07

signalword

Danger

Hazard Classifications

Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Carc. 1B - Eye Irrit. 2 - Muta. 1B - Repr. 2 - Skin Irrit. 2 - Skin Sens. 1 - STOT RE 1 Oral

target_organs

Peripheral nervous system

Storage Class

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

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Certificates of Analysis (COA)

Lot/Batch Number
0000454024
0000454024
0000474432
0000474432
0000386704

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Comparative performance of a novel herpes simplex virus type 2-specific enzyme-linked immunosorbent assay using a targeted chain oligopeptide, peptide 55
Al-Sulaiman AM, et al.
Clinical and Vaccine Immunology : CVI, 16(6), 931-934 (2009)
The
Ana Techniqs in Biotechnology (2011)
Kei Ihira et al.
Oncotarget, 8(8), 13509-13520 (2017-01-16)
EZH2 inhibition and reactivation of tumor suppressor microRNAs (miRNAs) represent attractive anti-cancer therapeutic strategies. We found that EZH2-suppressed let 7b and miR-361, two likely tumor suppressors, inhibited endometrial cancer (EC) cell proliferation and invasion, and abrogated cancer stem cell-like properties.
Preparation and properties of acrylamide gel for use in electrophoresis.
Raymond S and Wang YJ
Analytical Biochemistry, 1(4-5), 391-396 (1960)
Azis Boing Sitanggang et al.
Journal of food science and technology, 57(12), 4660-4670 (2020-10-23)
The influences of coagulation conditions on the characteristics of tofu have been investigated by many studies, with limited perspectives on the utilization of organic acid coagulants. Hence, this research aimed to study the psychochemical and functional properties of tofu coagulated
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Data Sheet - A3449

Instructions

Global Trade Item Number

SKUGTIN
A3449-100ML04061833360934
A3449-5L04061837689376
A3449-5X100ML04061833360941

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