A3429 and A3179 are the same solution but in different package sizes. No dilution is required for these products, but some customers may prefer a slightly weaker acid differentiation solution. The differentiation time may vary due to staining time and the thickness of the tissue sections being stained.
A3429
Differentiation Solution
for the differentiation of regressive hematoxylin stains
Synonym(s):
Acid Alcohol
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4 L
$152.00
$152.00
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4 L
$152.00
About This Item
$152.00
List price$160.00Save 5%Web-Only Promotion
Available to ship onApril 08, 2025Details
Request a Bulk Order
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form
solution
shelf life
40 mo.
color
colorless
application(s)
hematology
histology
storage temp.
room temp
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General description
Differentiation solution comprises acidified ethanol or isopropanol that is used to decolorize hematoxylin stain. The duration of exposure to the solution determines the degree of decolorization. Differentiation is a crucial step in hematoxylin-eosin staining to achieve the proper color contrast necessary for visualization, by selectively removing excess hematoxylin from the tissue section and leaving behind the desired staining intensity.[1]
Application
Acidified alcohol solution for the differentiation of regressive hematoxylin stains.
Features and Benefits
- Desired staining patterns can be obtained by varying the degree of exposure to the differentiation solution.
- Results are sharper and more rapid compared to other differentiation methods.[4]
- Crisp and clear differentiation between cellular structures is obtainable when performed according to Sigma-Aldrich Procedure No. GHS.
Principle
In regressive hematoxylin staining, a highly concentrated hematoxylin solution is used to rapidly overstain both nuclei and cytoplasm, and the cytoplasm is subsequently decolorized and excess hematoxylin is removed from the nucleus with dilute acid. Improper differentiation leaves excess residual hematoxylin that obscures the fine details of nuclear structures and chromatin and prevents eosin uptake.[1]
signalword
Danger
hcodes
Hazard Classifications
Eye Irrit. 2 - Flam. Liq. 2 - Met. Corr. 1 - STOT SE 2
target_organs
Eyes,Central nervous system
Storage Class
3 - Flammable liquids
wgk_germany
WGK 1
flash_point_f
71.6 °F
flash_point_c
22 °C
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Multispectral imaging as a tool for melanin detection
Kalleberg, et al.
Journal of Histotechnology, 38(1), 14-21 (2015)
Nuclear staining with alum hematoxylin
B D Llewellyn
Biotechnic & Histochemistry, 84(9), 159-177 (2009)
Oil Red O and Hematoxylin and Eosin Staining for Quantification of Atherosclerosis Burden in Mouse Aorta and Aortic Root
Andres-Manzano, et al.
Methods in Molecular Biology, 1339 (2015)
Pia M Teleman et al.
Acta obstetricia et gynecologica Scandinavica, 88(8), 927-932 (2009-07-07)
To outline possible associations between urinary incontinence (UI) and serum levels of steroid hormones in middle-aged women. Community-based observational study. All women aged 50-59 living in the Lund area by December 1995 were invited to a screening procedure. Sixty-four percent
M Jesús Andrés-Manzano et al.
Methods in molecular biology (Clifton, N.J.), 1339, 85-99 (2015-10-09)
Methods for staining tissues with Oil Red O and hematoxylin-eosin are classical histological techniques that are widely used to quantify atherosclerotic burden in mouse tissues because of their ease of use, reliability, and the large amount of information they provide.
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Do Differentiation Solutions A3179 and A3429 need to be diluted before use?
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