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Anti-Human IgE−Alkaline Phosphatase antibody, Mouse monoclonal

Name: Anti-Human IgE−Alkaline Phosphatase antibody, Mouse monoclonal
Brand: SIGMA
Price: 246 USD
Availability: InStock

clone GE-1, purified from hybridoma cell culture

Synonym(s):

Monoclonal Anti-Human IgE, Monoclonal Anti-Human IgE−Alkaline Phosphatase antibody produced in mouse

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Pack Size	SKU	Availability	Price
0.2 mL		Available to ship TODAYfromMILWAUKEE	$246.00
0.5 mL		Available to ship TODAYfromMILWAUKEE	$491.00

About This Item

NACRES:

NA.46

UNSPSC Code:

12352203

Conjugate:

alkaline phosphatase conjugate

Clone:

GE-1, monoclonal

Application:

DB, ELISA (d)

Citations:

$246.00

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biological source

mouse

Quality Level

200

conjugate

alkaline phosphatase conjugate

antibody form

purified from hybridoma cell culture

antibody product type

secondary antibodies

clone

GE-1, monoclonal

form

buffered aqueous solution

species reactivity

human

technique(s)

direct ELISA: 1:6,000, dot blot: 1:1,000

isotype

IgG2b

Related Categories

Secondary Antibodies

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1 of 1

This Item	A9544	B6775	I6760
Sigma-Aldrich A3076 Anti-Human IgE−Alkaline Phosphatase antibody, Mouse monoclonal	Sigma-Aldrich A9544 Anti-Human IgG (Fc specific)−Alkaline Phosphatase antibody produced in goat	Sigma-Aldrich B6775 Anti-Human IgG1−Biotin antibody, Mouse monoclonal	Sigma-Aldrich I6760 Monoclonal Anti-Human IgG (Fc specific) antibody produced in mouse
biological source mouse	biological source goat	biological source mouse	biological source mouse
species reactivity human	species reactivity -	species reactivity -	species reactivity rabbit, sheep, horse (IgG), goat, human
technique(s) direct ELISA: 1:6,000, dot blot: 1:1,000	technique(s) direct ELISA: 1:55,000, dot blot: 1:40,000 (chemiluminescent), immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:150	technique(s) direct ELISA: 1:1,000	technique(s) indirect ELISA: 1:3,000
conjugate alkaline phosphatase conjugate	conjugate alkaline phosphatase conjugate	conjugate biotin conjugate	conjugate unconjugated
antibody form purified from hybridoma cell culture	antibody form affinity isolated antibody	antibody form purified from hybridoma cell culture	antibody form ascites fluid
isotype IgG2b	isotype -	isotype IgG2a	isotype IgG2a

General description

Binds human IgE; does not bind human IgG, IgA or IgM.

IgEs are glycoprotein antibodies that regulate immunological responses to allergies and infections. These immunoglobulins have been implicated in mediating Type I hypersensitivity reactions. Elevated IgE levels have been associated with hyper IgE syndrome and allergic asthma.

Monoclonal Anti-Human IgE (mouse IgG2b isotype) is derived from the GE-1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from an immunized mouse.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Mouse monoclonal anti-human IgE-alkaline phosphatase antibody has been used for :

immunoblot assays
direct ELISA
dot blot

To confirm that a chimaeric monoclonal antibody had episolon chain, an elisa was performed using monoclonal anti-human IgE antibody (clone GE1) at a 1:1000 dilution.

Biochem/physiol Actions

Physical form

Solution in 0.05 M Tris, pH 8.0, containing 1% bovine serum albumin, 1 mM MgCl₂ and 15 mM sodium azide

Analysis Note

The antibody is tested for reactivity against myeloma IgE and allergy-elevated IgE.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class

10 - Combustible liquids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Class I chitinases with hevein-like domain, but not class II enzymes, are relevant chestnut and avocado allergens.

A Diaz-Perales et al.

The Journal of allergy and clinical immunology, 102(1), 127-133 (1998-07-29)

Several foods associated with the latex-fruit syndrome present relevant allergens of around 30 kd. Neither these components nor any other responsible for the reported cross-reactions have been identified and purified. We sought to isolate and characterize the 30 kd allergens

Allergenic properties of Polish nectar honeys.

Marta Burzyńska et al.

Acta scientiarum polonorum. Technologia alimentaria, 19(1), 15-24 (2020-04-01)

Small amounts of protein can be found in honey, including well known allergen sources, such as plant pollen and honeybee secretions. Despite this, there are few case reports describing allergic reactions following the consumption of honey. The aim of this

Lymphocyte Responses to Chymotrypsin- or Trypsin V-Digested beta-Lactoglobulin in Patients with Cow's Milk Allergy.

Masashi Kondo et al.

Allergy, asthma, and clinical immunology : official journal of the Canadian Society of Allergy and Clinical Immunology, 3(1), 1-9 (2007-03-15)

: Chymotrypsin- or trypsin V- (a mixture of trypsin and chymotrypsin) digested beta-lactoglobulin (BLG) peptides were prepared and were confirmed to have much less immunoglobulin (lg)G and lgE reactivity compared with intact BLG by IgG inhibition enzymelinked immunosorbent assay and

Exposure to allergens of different cattle breeds and their relevance in occupational allergy.

Astrid R R Heutelbeck et al.

International archives of occupational and environmental health, 82(9), 1123-1131 (2009-02-25)

Cattle are an important source of allergens in the working area of farmers. Asthma caused by cow allergens is a significant occupational problem. Yet in allergological testing, the results of in vivo and in vitro diagnostic tests are often inconsistent

Epitope Mapping of Rhi o 1 and Generation of a Hypoallergenic Variant: A CANDIDATE MOLECULE FOR FUNGAL ALLERGY VACCINES.

Gaurab Sircar et al.

The Journal of biological chemistry, 291(34), 18016-18029 (2016-07-01)

Efficacy of allergen-specific immunotherapy is often severely impaired by detrimental IgE-mediated side effects of native allergen during vaccination. Here, we present the molecular determinants for IgE recognition of Rhi o 1 and eventually converting the allergen into a hypoallergenic immunogen

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Global Trade Item Number

SKU	GTIN
A3076-.5ML	04061833360279
A3076-.2ML	04061833360262

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