A1970

Anti-VSV-Glycoprotein−Agarose antibody, Mouse monoclonal

clone P5D4, purified from hybridoma cell culture, PBS suspension

• Synonym(s): Monoclonal Anti-VSV Glycoprotein
• NACRES: NA.56
• UNSPSC Code: 12352203
• Conjugate: agarose conjugate
• Clone: P5D4, monoclonal
• Application: IP, protein purification
• Citations: 7

Properties

• biological source: mouse
• Quality Segment: 200
• conjugate: agarose conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: P5D4, monoclonal
• form: PBS suspension
• analyte chemical class(es): proteins (VSV-G)
• technique(s): immunoprecipitation (IP): suitable, protein purification: suitable
• isotype: IgG1
• capacity: ≥15 nmol/mL, resin binding capacity (VSV-G tagged fusion protein)
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Anti-VSV-G Agarose Conjugate is the immunoglobulin fraction of Monoclonal Anti-VSV glycoprotein covalently linked to agarose.

Immunogen

synthetic peptide containing the 15 carboxy-terminal amino acids (497-511) of Vesicular Stomatitis Virus Glycoprotein (VSV-G), conjugated to KLH.

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunoprecipitation (1 paper)

For immunoprecipitation and affinity purification of VSV-G tagged fusion proteins. The antibody recognizes an epitope containing the five carboxy-terminal amino acids of VSV Glycoprotein.

Biochem/physiol Actions

The antibody recognizes an epitope containing the five carboxy-terminal amino acids of VSV Glycoprotein. In infected cells, the antibody localizes the immature forms of VSV-G in the rough endoplasmic reticulum (RER) and in the cisternae of Golgi complex, as well as mature VSV-G at the cell surface and in the budding virus. The antibody does not stain the secreted form of VSV-G which lacks the membrane and the cytoplasmic domain. This antibody has been used for studies on the role of the cytoplasmic domain on newly-synthesized VSV-G during transfer to the plasma membrane and cell surface, using micro-injected antibody, immunoblotting, immunoprecipitation, immunocytochemistry and immunoelectron microscopy. The antibody has been used for the detection, immunoprecipitation and immunocytochemical staining of exogenously introduced constructs tagged with the carboxyl-terminus of VSV-G. This tag does not interfere with the function of the studied protein and can be specifically recognized by the P5D4 antibody without cross-reaction with any endogenous protein.

Physical form

Supplied as a suspension (1:1,v/v) of beaded agarose in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative

Preparation Note

Prepared using cyanogen bromide-activated agarose.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable