A0185

Monoclonal Anti-Biotin−Peroxidase antibody produced in mouse

clone BN-34, purified immunoglobulin

• Synonym(s): Monoclonal Anti-Biotin
• NACRES: NA.46
• UNSPSC Code: 12352203
• Conjugate: peroxidase conjugate
• Clone: BN-34, monoclonal
• Application: DB, ELISA (i), FACS, IHC (p), WB CL
• Citations: 21

Properties

• Quality Segment: 200
• biological source: mouse
• conjugate: peroxidase conjugate
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: BN-34, monoclonal
• packaging: vial of 0.5 mL
• technique(s): dot blot: suitable, flow cytometry: suitable, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:300, indirect ELISA: 1:30,000, western blot (chemiluminescent): 1:80,000
• isotype: IgG1
• storage temp.: 2-8°C
• target post-translational modification: unmodified

Description

General description

The antibody recognizes free biotin and biocytin using competitive ELISA. Using indirect ELISA or immuno­histo­chemical staining, the antibody also recognizes biotin conjugated to various immunoglobulins. Specificity is evaluated using biotinylated goat antibodies to human and rabbit antigens coated on multiwell plates.

Monoclonal Anti-Biotin (mouse IgG1 isotype) has been derived from the BN-34 hybridoma produced by the used successfully for detection of micro-injected, fusion of mouse myeloma cells and splenocytes from biotin-haptenized cytoskeletal proteins which enable BALB/c mice immunized with biotinylated keyhole limpet hemocyanin (KLH).

Immunogen

Biotin-KLH conjugate

Application

May be substituted for avidin to decrease non-specific binding.

Monoclonal Anti-Biotin Peroxidase antibody produced in mouse has been used:

• in immunohistochemistry
• in western blot
• as secondary antibody to detect the presence of aptamer on the membrane

The antibody may be used in amplification techniques, ELISA, immunoblotting, in situ nucleic acid hybridization, flow cytometry, image analysis or confocal microscopy.
In some applications, localization of biotinylated probes with avidin produces high background levels. Anti-biotin reagents may be substituted for avidin to decrease non-specific binding.

Biochem/physiol Actions

Biotin is an essential vitamin required for cells in living organisms and in cultures. The high binding affinity of biotin to egg white avidin or bacteria-derived streptavidin has been used to design immunoassays for detecting antigen-antibody interactions. The use of monoclonal anti-biotin IgG can enhance the sensitivity of such avidin-biotin immunoassays. The monoclonal antibody functions by selectively enlarging and bridging the avidin-biotin-enzyme complexes, thereby increasing the signals from substrate conversion. Furthermore, this antibody can be used in many other applications where biotin may be introduced as target label.
Monoclonal Anti-Biotin-Peroxidase antibody specific for biotin conjugated to various proteins. The antibody recognizes free biotin and biocytin using competitive ELISA. Using indirect ELISA or immuno­histo­chemical staining, the antibody also recognizes biotin conjugated to various immunoglobulins. The product specificity has been evaluated using biotinylated goat antibodies to human and rabbit antigens coated on multiwell plates.

Physical form

Lyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 0.05% MIT

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H317
• pcodes: P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364
• Hazard Classifications: Skin Sens. 1
• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable