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Sigma-Aldrich

2-Propanol

≥99.5% (GC), for molecular biology, BioUltra

Synonym(s):

sec-Propyl alcohol, IPA, Isopropanol, Isopropyl alcohol

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About This Item

Linear Formula:
(CH3)2CHOH
CAS Number:
67-63-0
Molecular Weight:
60.10
Beilstein/REAXYS Number:
635639
EC Number:
200-661-7
MDL number:
MFCD00011674
UNSPSC Code:
12352104
PubChem Substance ID:
57651672
NACRES:
NA.25

product name

2-Propanol, BioUltra, for molecular biology, ≥99.5% (GC)

grade

for molecular biology

Quality Level

200

vapor density

2.1 (vs air)

vapor pressure

33 mmHg ( 20 °C)
44 mmHg ( 25 °C)

product line

BioUltra

assay

≥99.5% (GC)

form

liquid

autoignition temp.

750 °F

expl. lim.

2.0-12.7 %, 93 °C

technique(s)

DNA extraction: suitable
RNA extraction: suitable

impurities

DNases, none detected
 RNases, none detected
 insoluble matter, passes filter test
 phosphatases, none detected
 proteases, none detected

evapn. residue

≤0.001%

color

colorless

refractive index

n20/D 1.377 (lit.)
n20/D 1.378

bp

82 °C (lit.)

mp

−89.5 °C (lit.)

solubility

water: soluble (completely)

density

0.785 g/mL at 25 °C (lit.)

cation traces

Al: ≤0.5 mg/kg
Ba: ≤0.1 mg/kg
Bi: ≤0.1 mg/kg
Ca: ≤0.5 mg/kg
Cd: ≤0.05 mg/kg
Co: ≤0.02 mg/kg
Cr: ≤0.02 mg/kg
Cu: ≤0.02 mg/kg
Fe: ≤0.1 mg/kg
K: ≤0.5 mg/kg
Li: ≤0.1 mg/kg
Mg: ≤0.1 mg/kg
Mn: ≤0.02 mg/kg
Mo: ≤0.1 mg/kg
Na: ≤0.5 mg/kg
Ni: ≤0.02 mg/kg
Pb: ≤0.1 mg/kg
Sr: ≤0.1 mg/kg
Zn: ≤0.1 mg/kg

SMILES string

CC(C)O

λ

neat

UV absorption

λ: 260 nm Amax: 0.02
λ: 280 nm Amax: 0.01

suitability

suitable for molecular biology

InChI

1S/C3H8O/c1-3(2)4/h3-4H,1-2H3

InChI key

KFZMGEQAYNKOFK-UHFFFAOYSA-N

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General description

2-propanol is a clear, colorless, polar organic solvent commonly used in chemical and biochemical laboratories. It will dissolve a wide range of chemicals, and evaporates quickly. Isopropanol is commonly used in molecular biology for DNA and RNA extraction through precipitation. Unlike ethanol, which requires 2.5 times the volume of the nucleic acid-containing sample, isopropanol can be added in an equal volume. This causes precipitation to occur even without cooling, making it advantageous in reducing the overall volume of the sample. Moreover, it has applications in quantifying hydroxyproline levels, assessing cell viability through MTT assays, and contributing to microarray hybridization processes.

application

2-Propanol has been used:
  • to precipitate genomic DNA extracted from flies
  • to precipitate genomic DNA extracted from xenograft primary tumors and organs
  • to immunoprecipitate 5-bromo-2-deoxyuridine (BrdU)

Biochem/physiol Actions

Isopropanol (2-Propanol) is widely used in molecular biology for the precipitation of DNA during extraction from cells and tissues. BioUltra IPA is suitable for DNA precipitation.

Features and Benefits

  • Suitable as a reagent for Biochemical and Molecular Biology Research
  • BioUltra grade, meets strict purity standards through rigorous testing
  • Free from DNase, NICKase, RNase, and Protease
  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications

Other Notes

For additional information on our range of Biochemicals, please complete this form.

comparable product

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pictograms

FlameExclamation mark
GHS02,GHS07

signalword

Danger

Hazard Classifications

Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3

target_organs

Central nervous system

Storage Class

3 - Flammable liquids

wgk_germany

WGK 1

flash_point_f

53.6 °F - closed cup

flash_point_c

12.0 °C - closed cup

ppe

Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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