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MilliporeSigma

02744

Atto 655-Streptavidin

BioReagent, suitable for fluorescence

Synonym(s):

Streptavidin-Atto 655

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1 MG

$420.00

$420.00

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About This Item

NACRES:
NA.32
UNSPSC Code:
12352116

Key Documents

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product line

BioReagent

form

powder

manufacturer/tradename

ATTO-TEC GmbH

solubility

DMF: soluble, DMSO: soluble, acetonitrile: soluble

fluorescence

λex 655 nm; λem 680 nm in 0.1 M phosphate pH 7.0

suitability

suitable for fluorescence, suitable for gel electrophoresis

storage temp.

−20°C

Quality Level

100

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This Item
0926006966RSTREP-RO
Sigma-Aldrich

Sigma-Aldrich

02744

Atto 655-Streptavidin

Sigma-Aldrich

Sigma-Aldrich

09260

Atto 425-Streptavidin

Sigma-Aldrich

Sigma-Aldrich

06966

Atto 655-Biotin

Streptavidin, recombinant liquid, ≥17 units/mg protein, pH range 5.0-9.0

Roche

RSTREP-RO

Streptavidin, recombinant

suitability

suitable for fluorescence, suitable for gel electrophoresis

suitability

-

suitability

suitable for fluorescence

suitability

suitable for immunological tests

fluorescence

λex 655 nm; λem 680 nm in 0.1 M phosphate pH 7.0

fluorescence

λex 437 nm; λem 477 nm in 0.1 M phosphate pH 7.0

fluorescence

λex 655 nm; λem 680 nm in 0.1 M phosphate pH 7.0

fluorescence

-

solubility

DMF: soluble, acetonitrile: soluble, DMSO: soluble

solubility

H2O: soluble

solubility

DMF: soluble, DMSO: soluble, acetonitrile: soluble

solubility

water: 10 mg/L (mA405=100)

form

powder

form

powder

form

powder

form

liquid

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

-10 to -25°C

Application

Atto 655 is a fluorescent label with high molecular absorption (125.000) and quantum yield (0.30), as well as sufficient stoke′s shift (excitation maximum 663 nm, emission maximum 684 nm). There is very little cis-trans-isomerisation, which limits brightness and reproducibility for many other dyes. Atto 655 has been coupled to streptavidin to enable various types of assays based on the specific affinity of biotin to streptavidin. Streptavidin conjugates are used as secondary detection reagents in histochemical applications, flow cytometry, microarrays, blot analysis and immunoassays.

Analysis Note

free of unconjugated dye

Legal Information

This product is for Research use only. In case of intended commercialization, please contact the IP-holder (ATTO-TEC GmbH, Germany) for licensing.

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCM6542
BCCL7578
BCCK3312
BCCJ3542
BCCF1140

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Breaking the Diffraction Barrier in Fluorescence Microscopy by Optical Shelving.
Bretschneider, S.; Eggeling, Chr.; Hell, S. W.
Physical Review Letters, 98(21) (2007)
Triple-Color Super-Resolution Imaging of Live Cells. Resolving Submicroscopic Receptor Organization in the Plasma Membrane.
Wilmes, S.; Staufenbiel, M.; Lisse, D.; Richter, Ch. P.; Beutel, O.; Busch, K. B. et al.
Angewandte Chemie (International Edition in English), 124(20), 4952-4955 (2012)
Simple buffers for 3D STORM microscopy.
van de Linde, S.; Kasper, R.; Heilemann, M.; Sauer, M.
Applied Physics B: Lasers and Optics, 93(4), 725-731 (2008)
PALM and STORM. Unlocking live-cell super-resolution.
Henriques, R.; Griffiths, C.; Hesper Rego, E.; Mhlanga, Musa M.
Biopolymers, 95(5), 322-331 (2011)
Direct stochastic optical reconstruction microscopy with standard fluorescent probes.
van de Linde, S.; et al.
Nature Protocols, 6(7), 991-1009 (2011)
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