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Millipore

TCBS Agar

NutriSelect® Plus, suitable for microbiology

Synonym(s):

Vibrio Selective Agar, Thiosulfate Citrate Bile Sucrose Agar

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About This Item

UNSPSC Code:
41171606
NACRES:
NA.74

agency

according to ISO 21872-1:2017
according to ISO 21872-2:2007

Quality Level

200

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 14 g/L
bromothymol blue, 0.04 g/L
ferric citrate, 1 g/L
mixed peptone, 10 g/L
ox-bile, 5 g/L
sodium chloride, 10 g/L
sodium cholate, 3 g/L
sodium citrate, 10 g/L
sodium thiosulfate, 10 g/L
sucrose, 20 g/L
thymol blue, 0.04 g/L
yeast extract, 5 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

8.6±0.2 (25 °C)

application(s)

environmental
food and beverages
microbiology

suitability

selective and differential for Vibrio spp.

Related Categories

General description

Thiosulfate citrate bile salts sucrose (TCBS) Agar uses an alkaline pH (8.6), ox bile and 1% NaCl to prevent the growth of gram positive bacteria, members of the Enterobacteriaceae family and the genus Pseudomonas.

Application

Thiosulfate citrate bile salts sucrose (TCBS) Agar is a selective agar for the isolation and cultivation of Vibrio cholerae and other Vibrio species.

Preparation Note

Dissolve 88 g in 1 litre distilled water and bring to the boil. Pour plates. Do NOT autoclave.

Other Notes

Incidence of Vibrio cholerae from estuaries of the United States West Coast; Survival and multiplication of Vibrio cholerae serotype Ogawa in reconstituted infant milk

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

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Certificates of Analysis (COA)

Lot/Batch Number
BCCK9360
BCCJ8262
BCCK3269
BCCH0274
BCCF9771

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C A Kaysner et al.
Applied and environmental microbiology, 53(6), 1344-1348 (1987-06-01)
The incidence of Vibrio cholerae in shellfish, sediment, and waters of California, Oregon, and Washington was determined during the summer of 1984. Samples from 24 distinct estuaries were analyzed qualitatively. V. cholerae non-O1 was found in 23 estuaries and in
Dongyou Liu
Molecular Detection of Human Bacterial Pathogens (2011)
Asmaa T Y Kishawy et al.
Animals : an open access journal from MDPI, 10(10) (2020-09-27)
The aim of the present study was to evaluate mannan oligosaccharides (MOS) or glycerol (GLY) as a carbon source on biofloc systems of Nile tilapia (O. niloticus) juveniles. Fish (n = 750) were reared in open flow (Controls) or biofloc
M.O. Odugbo et al.
Journal of Food Protection, 53, 1071-1071 (1990)

Articles

Vibrio Detection

Vibrios are motile, curved or comma-shaped bacilli and have a single polar flagella with sheet proteins. They are often found in open water, freshwater and saltwater.

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