NGLYF-RO

N-Glycosidase F

PNGase F of Flavobacterium meningosepticum, recombinant from E. coli

• Synonym(s): glycosidase
• UNSPSC Code: 12352204
• EC Number: 3.5.1.52 (BRENDA, IUBMB)
• NACRES: NA.54
• Specific activity: ~25000 units/mg protein
• Assay: ≥90% (SDS-PAGE)
• Recombinant: expressed in E. coli

Properties

• recombinant: expressed in E. coli
• conjugate: (N-linked)
• assay: ≥90% (SDS-PAGE)
• form: solution
• specific activity: ~25000 units/mg protein
• packaging: pkg of 0.1 mL (11365169001 [100 U]), pkg of 0.25 mL (11365177001 [250 U])
• manufacturer/tradename: Roche
• optimum pH: 7.0-8.0
• storage temp.: −20°C

Description

General description

Peptide-N-glycosidase F, Peptide-N⁴-(acetyl-β-glucosaminyl)-asparagine amidase

Application

Use N-glycosidase F to cleave all types of asparagine-bound N-glycans, provided that the amino group as well as the carboxyl group are present in a peptide linkage, and that the oligosaccharide has the minimum length of the chitobiose core unit.[1][2][3] The reaction products are ammonia, aspartic acid (in the peptide chain), and the complete oligosaccharide.
Note: N-Glycosidase F, recombinant is also available as a lyophilizate without glycerol.

Physical form

Solution in 50 mM sodium phosphate, 12.5 mM EDTA, 50% glycerol (v/v), pH 7.2

Other Notes

For life science research only. Not for use in diagnostic procedures.

One unit is the enzyme activity which hydrolyzes 1 nmol dabsyl fibrin glycopeptide or 0.2 nmol dansyl fetuin glycopeptide within 1 minute at 37 °C at pH 7.8.

Legal Information

The sale of the Product does not exhaust or grant any rights in third party patents including patents of companies of the F. Hoffmann - La Roche AG group of companies, in particular, for the use of modified antibodies obtained by using the product.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: No data available
• flash_point_c: No data available