DGTP-RO

dGTP

PCR Grade, sodium salt

• Synonym(s): 2′-Deoxyguanosine 5′-triphosphate trisodium salt solution, dGTP-Na₃
• Empirical Formula (Hill Notation): C₁₀H₁₃N₅Na₃O₁₃P₃
• CAS Number: 93919-41-6
• Molecular Weight: 573.13
• MDL number: MFCD00080337
• UNSPSC Code: 12352302
• PubChem Substance ID: 329798919

Properties

• Quality Level: 100
• assay: 99% (dNTP)
• form: solution
• usage: sufficient for 125,000 standard reactions (03732703001); sufficient for 31,250 standard reactions (11969030001); sufficient for 6,250 standard reactions (11934538001)
• packaging: pkg of 1,250 μL (11969030001 [100 mM]); pkg of 4 × 1,240 μL (03732703001 [4x100 mM]); pkg of 250 μL (11934538001 [100 mM])
• manufacturer/tradename: Roche
• concentration: <0.9% (dNDP); 100 mM
• storage temp.: −20°C
• SMILES string: [Na+].[Na+].[Na+].NC1=Nc2c(ncn2[C@H]3C[C@H](O)[C@@H](COP([O-])(=O)OP([O-])(=O)OP(O)([O-])=O)O3)C(=O)N1
• InChI: 1S/C10H16N5O13P3.3Na/c11-10-13-8-7(9(17)14-10)12-3-15(8)6-1-4(16)5(26-6)2-25-30(21,22)28-31(23,24)27-29(18,19)20;;;/h3-6,16H,1-2H2,(H,21,22)(H,23,24)(H2,18,19,20)(H3,11,13,14,17);;;/q;3*+1/p-3/t4-,5+,6+;;;/m0.../s1
• InChI key: IWGGLKOTEOCWQP-BIHLCPNHSA-K

Description

General description

2′-Deoxyguanosine 5′-triphosphate (dGTP) is made of a guanine nucleobase attached to deoxyribose and a 5′-hydroxyl on the sugar linked to a chain of three phosphate residues. It is used by the cells for the synthesis of DNA with the help of DNA polymerase.

dGTP, PCR Grade, is a 100 mM clear, colorless solution of the sodium salt (pH 8.3). PCR Grade nucleotides from Roche are specially manufactured and purified to the highest possible chemical purity. PCR Grade nucleotides contain at least 99% of the relevant deoxynucleoside-triphosphate (dNTP) and less than 0.9% deoxynucleoside-diphosphate (dNDP).

Application

2′-Deoxyguanosine 5′-triphosphate trisodium salt is utilized for methods such as DNA sequencing, PCR and other DNA polymerase based DNA synthesis and repair techniques.

Quality

Function tested in RT-PCR. Tested for absence of contaminating deoxyribonucleases, ribonucleases and nicking activities according to the current Quality Control procedures.

Physical form

solution of dGTP in water (pH 8.3).

Preparation Note

Working solution: We recommend to prepare a deoxynucleotide mix containing dATP, dCTP, dGTP, dTTP (10 mM, each); (e.g., for the preparation of 100 μl nucleotide mix add 10 μl of dATP, dCTP, dGTP, dTTP (each) to 60 μl Water, PCR Grade).

Other Notes

For general laboratory use.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 1
• flash_point_f: does not flash
• flash_point_c: does not flash