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SF006

ESGRO Complete Accutase

The ESGRO Complete Accutase is a cell detachment solution of proteolytic & collagenolytic enzymes, qualified for use for the detachment of mouse embryonic stem cells cultured in serum-free conditions with ESGRO Complete Clonal Grade Medium.

Synonym(s):

Stem Cell Tested Accutase

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Size/SKUAvailabilityPrice
100 mL
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$57.90

About This Item

UNSPSC Code:
12352207
NACRES:
NA.75
eCl@ss:
32160801

$57.90

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Quality Segment

100

form

liquid

manufacturer/tradename

Chemicon®

technique(s)

cell culture | stem cell: suitable

input

sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)

shipped in

dry ice

storage temp.

-10 to -25°C

General description

ESGRO Complete™ Accutase is a cell detachment solution of proteolytic and collagenolytic enzymes that has been qualified for use for the detachment of mouse embryonic stem cells cultured in serum-free conditions with ESGRO Complete Clonal Grade Medium (Cat. No. SF001-500). Accutase does not contain mammalian or bacterial derived products.

Application

Cell Detachment:

1. Thaw Accutase® to room temperature.

2. Wash plate, flask or beads with sterile PBS.

3. Add Accutase to culture dish or flask using aseptic procedures at 10 mL per 75 cm2 surface area.

4. Return culture to 37°C incubator and allow cells to detach (5-10 minutes).

5. Count cells and passage as usual. No additional washes or enzyme inhibitors are required.

Physical form

Frozen sterile liquid, ready to use formulation. Each lot is tested for Sterility (by USP membrane filtration method), enzymatic activity (tested with synthetic chromagenic tetrapeptides) and cell detachment from tissue culture plastic.

1X Accutase® enzymes in Dulbecco′s PBS containing 0.5 mM EDTA•4Na and 3 mg/L Phenol Red.

Preparation Note

Stable when stored at -20°C. Refer to lot expiration date on label. Recommended storage upon receipt is -20°C. After thawing, Accutase® may be stored for up to 2 months at 4°C. DO NOT STORE AT ROOM TEMPERATURE.

Legal Information

Accutase is a registered trademark of Innovative Cell Technologies, Inc.
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
cOmplete is a trademark of Roche

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This Item
SCR005A6964SCR104
ESGRO Complete Accutase The ESGRO Complete Accutase is a cell detachment solution of proteolytic & collagenolytic enzymes, qualified for use for the detachment of mouse embryonic stem cells cultured in serum-free conditions with ESGRO Complete Clonal Grade Medium.

Sigma-Aldrich

SF006

ESGRO Complete Accutase

Accutase cell detachment solution A cell detachment solution of proteolytic & collagenolytic enzymes. The reagent is useful for creating single cell suspensions from clumped cell cultures for accurate cell counting, detachment of cells from primary tissue.

Millipore

SCR005

Accutase cell detachment solution

Accutase® solution sterile-filtered, suitable for cell culture

Sigma-Aldrich

A6964

Accutase® solution

Accutase®XL Lyophilized Accutase AccutaseXL is a lyophilized form of Accutase with extended stability used for the routine detachment of cells from standard tissue culture plasticware

Sigma-Aldrich

SCR104

Accutase®XL Lyophilized Accutase

Quality Level

100

Quality Level

300

Quality Level

200

Quality Level

300

technique(s)

cell culture | stem cell: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable, single cell analysis: suitable

technique(s)

cell culture | mammalian: suitable

input

sample type: mouse embryonic stem cell(s)
sample type induced pluripotent stem cell(s)

input

sample type cardiac stem cell(s)
sample type neural stem cell(s)
sample type pancreatic stem cell(s)
sample type: mouse embryonic stem cell(s)
sample type: human embryonic stem cell(s)
sample type mesenchymal stem cell(s)
sample type hematopoietic stem cell(s)
sample type induced pluripotent stem cell(s)
sample type epithelial cells

input

-

input

-

manufacturer/tradename

Chemicon®

manufacturer/tradename

Chemicon®

manufacturer/tradename

-

manufacturer/tradename

Chemicon®

storage temp.

-10 to -25°C

storage temp.

-10 to -25°C

storage temp.

−20°C

storage temp.

-10 to -25°C

form

liquid

form

liquid

form

liquid

form

liquid

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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