MABT843
Anti-SYNE1 Antibody, clone 7A12 (MANNES1A)
clone 7A12, from mouse
Synonym(s):
Nesprin-1, Enaptin, Myne-1, Myocyte nuclear envelope protein 1, Nuclear envelope spectrin repeat protein 1, Synaptic nuclear envelope protein 1, Syne-1
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
7A12, monoclonal
species reactivity
rat, human, mouse, rabbit
technique(s)
immunocytochemistry: suitable
western blot: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
ambient
target post-translational modification
unmodified
Gene Information
human ... SYNE1(23345)
General description
Nesprin-1 (UniProt Q8NF91; also known as Enaptin, Myne-1, Myocyte nuclear envelope protein 1, Nuclear envelope spectrin repeat protein 1, Synaptic nuclear envelope protein 1, Syne-1) is encoded by the SYNE1 (also known as C6orf98, EDMD4, KIAA0796, KIAA1262, KIAA1756, MYNE1, SCAR8) gene (Gene ID 23345) in human. SYNE1 gene encodes multiple intracellular linker/scaffold molecules as a result of alternate start codons usage and splicings. Structurally, nesprins have a central rod domain composed of spectrin repeats. Nesprin-1 isoforms giant/4/5/6/7/10 and nesprin-2 isoforms giant/p32CH/NUANCE-N-33 have two N-terminal CH (calponin homology) domains that bind the actin cytoskeleton. Nesprin-1 isoforms giant/beta/alpha/4/beta 2/alpha 2/myne-1 and Nesprin-2 isoforms giant/2/epsilon2/beta1/alpha1/alpha2/gamma/beta2/FLJ56122/FLJ55476/epsilon1 contain a C-terminal transmembrane KASH (Klarsicht-ANC-Syne-homology) domain that resides in the outer nuclear membrane and bind across the luminal space to the SUN (Sad1, UNC84) type II inner nuclear membrane proteins, SUN1 and SUN2, to form LINC (Linker of Nucleoskeleton and Cytoskeleton) complexes that link the cytoskeleton and the nucleus. Human SYNE1 gene is among the top ten loci conferring risk for the five major psychiatric disorders, schizophrenia, bipolar disorder (BD), major depression, attention deficit-hyperactivity disorder (ADHD), and autism spectrum disorder (ASD), as well as multiple other neurological and neuromuscular disorders.
Specificity
Epitope region is present in, human isoforms 1 (Giant; Enaptin)/2 (Beta)/3 (Alpha)/4/5/8 (Beta 2)/9 (Alpha 2)/11 (myne-1; 131kDa), mouse isoforms 1 (Giant; Enaptin)/2/3 (Syne-1A)/4 (Syne-1B)), and rat Nesprin-1 (UniProt Q8VHJ9), but absent from human spliced isoforms 6/7/10 (drop1)/12 (GSRP-56; 56 kDa), mouse spliced isoform 5 (Enaptin-165), or rat spliced isoform CPG2 (UniProt Q63128) reported by UniProt (Q8NF91, Q6ZWR6, Q8VHJ9, Q63128). Clone 7A12 does not reacts with nesprin-2, but stained centrosome in acetone/methanol-fixed HeLa cells due to uncharacterized cross-reactivity (Randles, K.N., et al. (2010). Dev. Dyn. 239(3):998-1009).
Immunogen
GST-tagged recombinant human SYNE1 isoform Beta C-terminal 121-a.a. fragment fused to full-length isoform Alpha sequence (UniProt Q8NF91-2 andQ8NF91-3).
Application
Research Category
Cell Structure
Cell Structure
This mouse monoclonal Anti-SYNE1, clone 7A12 (MANNES1A) , Cat. No. MABT843 is validated for use in and Immunocytochemistry, Western Blotting and Western Blotting, for the detection of Nesprin-1.
Western Blotting Analysis: 10 µg/mL from a representative lot detected SYNE1 spliced isoform Giant (Enaptin) in lysate from a 4-day old human skeletal myotube culture (Courtesy of Glenn E. Morris, Ph.D., Wolfson Centre for Inherited Neuromuscular Disease, Oswestry, United Kingdom).
Immunocytochemistry Analysis: A representative lot immunostained the nucleus of acetone/methanol-fixed rat aortic vascular smooth muscle cells (VSMCs) by fluorescent immunocytochemistry (Duong, N.T., et al. (2014). PLoS One. 9(4):e94380).
Immunocytochemistry Analysis: A representative lot detected SYNE1 immunoreactivity predominantly in the nucleus by fluorescent immunocytochemistry staining of acetone/methanol-fixed human skin fibroblasts regardless of their emerin expression. SYNE1 immunoreactivity was redistributed to ER in the absence of lamin A/C in Lamn-/- mouse embryonic fibroblasts (MEFs) (Randles, K.N., et al. (2010). Dev. Dyn. 239(3):998-1009).
Immunocytochemistry Analysis: A representative lot immunostained multinucleate myotube nuclear envelopes, while only very weak or little SYNE1 immunoreactivity was seen among mononucleated myoblasts in myoblast cultures undergoing differentiation (Randles, K.N., et al. (2010). Dev. Dyn. 239(3):998-1009).
Western Blotting Analysis: A representative lot detected multiple SYNE1 spliced isoforms (including Giant/Enaptin, Beta, and Alpha) in human muscle tissue, skeletal muscle cells and dermal fibroblasts, as well as in Lmna-/- mouse embryonic fibroblasts (MEFs) (Randles, K.N., et al. (2010). Dev. Dyn. 239(3):998-1009).
Immunocytochemistry Analysis: A representative lot immunostained the nucleus of acetone/methanol-fixed rat aortic vascular smooth muscle cells (VSMCs) by fluorescent immunocytochemistry (Duong, N.T., et al. (2014). PLoS One. 9(4):e94380).
Immunocytochemistry Analysis: A representative lot detected SYNE1 immunoreactivity predominantly in the nucleus by fluorescent immunocytochemistry staining of acetone/methanol-fixed human skin fibroblasts regardless of their emerin expression. SYNE1 immunoreactivity was redistributed to ER in the absence of lamin A/C in Lamn-/- mouse embryonic fibroblasts (MEFs) (Randles, K.N., et al. (2010). Dev. Dyn. 239(3):998-1009).
Immunocytochemistry Analysis: A representative lot immunostained multinucleate myotube nuclear envelopes, while only very weak or little SYNE1 immunoreactivity was seen among mononucleated myoblasts in myoblast cultures undergoing differentiation (Randles, K.N., et al. (2010). Dev. Dyn. 239(3):998-1009).
Western Blotting Analysis: A representative lot detected multiple SYNE1 spliced isoforms (including Giant/Enaptin, Beta, and Alpha) in human muscle tissue, skeletal muscle cells and dermal fibroblasts, as well as in Lmna-/- mouse embryonic fibroblasts (MEFs) (Randles, K.N., et al. (2010). Dev. Dyn. 239(3):998-1009).
Quality
Identity Confirmation by Isotyping Test.
Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1 .
Isotyping Analysis: The identity of this monoclonal antibody is confirmed by isotyping test to be mouse IgG1 .
Target description
~1,000 kDa (Giant; Enaptin) observed. 1,011/380.4/112.4/1,005/167.3/367.9/111.6/131.2 kDa (human isoforms 1 (Giant; Enaptin)/2 (Beta)/3 (Alpha)/4/5/8 (Beta 2)/9 (Alpha 2)/11 (myne-1; 131kDa)), 1010/111,3/108.8/1010 kDa (mouse isoforms 1 (Giant; Enaptin)/2/3 (Syne-1A)/4 (Syne-1B)), and 325.1 kDa (rat Nesprin-1; UniProt Q8VHJ9) calculated. Uncharacterized bands may be observed in some lysate(s).
Physical form
Format: Purified
Protein G purified.
Purified mouse IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
12 - Non Combustible Liquids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Li-Chun Cheng et al.
Life science alliance, 6(9) (2023-07-12)
The nuclear envelope (NE) is a subdomain of the ER with prominent roles in nuclear organization, which are largely mediated by its distinctive protein composition. We developed methods to reveal low-abundance transmembrane (TM) proteins concentrated at the NE relative to
Qiao Zhang et al.
Current biology : CB, 29(17), 2826-2839 (2019-08-14)
The nucleoskeleton and cytoskeleton are important protein networks that govern cellular behavior and are connected together by the linker of nucleoskeleton and cytoskeleton (LINC) complex. Mutations in LINC complex components may be relevant to cancer, but how cell-level changes might
Li-Chun Cheng et al.
Nucleus (Austin, Tex.), 10(1), 126-143 (2019-05-31)
The double membrane nuclear envelope (NE), which is contiguous with the ER, contains nuclear pore complexes (NPCs) - the channels for nucleocytoplasmic transport, and the nuclear lamina (NL) - a scaffold for NE and chromatin organization. Since numerous human diseases linked to NE
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