MABT1475

Anti-Cytokeratin-8 Antibody, clone 1E8

• Synonym(s): CYK8, KRT8, Keratin, type II cytoskeletal 8, Keratin-8 (K8), Type-II keratin Kb8
• UNSPSC Code: 12352203

Properties

• biological source: mouse
• Quality Level: 200
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 1E8, monoclonal
• mol wt: calculated mol wt 53.7 kDa; observed mol wt ~54 kDa
• purified by: using protein G
• species reactivity: human
• species reactivity (predicted by homology): canine, monkey
• packaging: antibody small pack of 100
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable; inhibition assay: suitable; western blot: suitable
• isotype: IgG2aκ
• epitope sequence: C-terminal
• Protein ID accession no.: NP_001243222
• UniProt accession no.: P05787
• storage temp.: -10 to -25°C
• Gene Information: human ... KRT8(3856)

Description

Specificity

Clone 1E8 is a mouse monoclonal antibody that detects human Cytokeratin-8. It targets an epitope within 12 amino acids from the C-terminal part.

Immunogen

KLH-conjugated linear peptide corresponding to 12 amino acids from the C-terminal of human Cytokeratin-8.

Application

Quality Control Testing

Evaluated by Western Blotting in HaCaT cell lysate.

Western Blotting Analysis: A 1:500 dilution of this antibody detected Cytokeratin-8 in HaCaT cell lysate.

Tested Applications

Immunohistochemistry Applications: A representative lot detected Cytokeratin-8 in Immunohistochemistry application (Sfakianos, J.P., et al. (2020). Nat Commun. 11(1):2540).

Western Blotting Analysis: A representative lot detected Cytokeratin-8 in Western Blotting application (Hembrough, T.A., et al. (1996). J Biol Chem. 271(41):25684-91).

Immunocytochemistry Analysis: A representative lot detected Cytokeratin-8 in Immunocytochemistry application (Hembrough, T.A., et al. (1996). J Biol Chem. 271(41):25684-91).

Inhibition Assay: An Anti-Cytokeratin-8, clone 1E8 Fab decreased plasminogen binding to breast cancer cell lines in Inhibition Assay (Hembrough, T.A., et al. (1996). J Biol Chem. 271(41):25684-91).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Target description

Keratin, type II cytoskeletal 8 (UniProt: P05787; also known as Cytokeratin-8, CK-8, Keratin-8, K8, Type-II keratin Kb8) is encoded by the KRT8 (also known as CYK8) gene (Gene ID: 3856) in human. Cytokeratins belong to a diverse group of intermediate filaments that participate in differentiation and maintain the structural integrity of epithelial cells. They share a common structural organization: a central -helical rod domain flanked by head and tail domains. An important unique feature of cytokeratins is their epithelial cell-type-specific expression. CK8 is expressed in abundance in the epithelia of colon, bladder, ileum, and stomach. It is a type II, neutral to basic protein that together with cytokeratin-19 (KRT19) helps to link the contractile apparatus to dystrophin at the costameres of striated muscle. CK-8 can undergo phosphorylation on three major serine residues: Serine 23, 431, and 73. Serine 23 is highly conserved in all type II keratins. Phosphorylation at serine 73 is reported to increase during cellular stress. However, under normal conditions serine 73 remains largely dephosphorylated. It can also undergo O-glycosylation in a cell cycle-dependent manner and glycosylation increases its solubility and reduces stability by inducing proteasomal degradation. CK8 expression has been correlated with malignancy in leukoplakia and carcinomas of the head and neck and its expression has been observed in all non-small-cell lung cancers.

Physical form

Purified mouse monoclonal antibody IgG2a in PBS without azide.

Reconstitution

0.5 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Storage and Stability

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable