MABF463

Anti-Foxp3 Antibody (mouse), FITC, clone 3G3

clone 3G3, 0.5 mg/mL, from mouse

• Synonym(s): Forkhead box protein P3, Scurfin
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: FITC conjugate
• Clone: 3G3, monoclonal
• Application: FACS
• Citations: -

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: FITC conjugate
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 3G3, monoclonal
• species reactivity: mouse
• concentration: 0.5 mg/mL
• technique(s): flow cytometry: suitable
• isotype: IgG1κ
• UniProt accession no.: Q99JB6
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: mouse ... Foxp3(20371)

Description

General description

The 3G3 antibody reacts with mouse Foxp3, a 50-55 kDa transcription factor which is a central regulator of T cell activity and is critical for the development and function of regulatory T cells (Tregs). Foxp3 is expressed at constitutively high levels in Treg cells, which are further identified as being CD4+ CD25+. In resting conventional T cells (CD4+ CD25-) Foxp3 expression is restricted, and upon TCR activation is expressed only transiently and in a small proportion of cells. However, the growth factor TGF-beta has been shown to induce expression of Foxp3 in naïve T cells, driving their development into Foxp3+ Tregs, which are called “induced” or “adaptive” Tregs. These cells are phenotypically similar to so-called “natural” Tregs (CD4+ CD25high Foxp3+) which originate in the thymus and comprise the majority of Treg cells. Tregs are critical for maintaining peripheral tolerance and are implicated in the development of autoimmunity.It is important to review the literature in choosing an antibody for the Foxp3 antigen in flow cytometry, as the potential for high background or non-specific staining may be observed. The 3G3 antibody may be used for intracellular detection of Foxp3 in cells from mouse and Rhesus macaque.

Immunogen

Immunogen information for this antibody is proprietary information.

Application

Research Category
Inflammation & Immunology

This mouse monoclonal antibody Anti-Foxp3 (mouse), FITC & clone 3G3 validated for use in Flow Cytometry for the detection of Foxp3.

Physical form

Purified Mouse Monoclonal Antibody in buffer containing 10 mM NaH2PO4, 150 mM NaCl, 0.05% BSA, pH8, and up to 0.1% sodium azide.

Size Exclusion Chromatography

Preparation Note

It is recommended to store the product undiluted at 2-8°C and protected from prolonged exposure to light. Do not freeze.

Analysis Note

The monoclonal antibody was purified from tissue culture supernatant via affinity chromatography. The purified antibody was conjugated under optimal conditions, with unreacted dye removed from the preparation.

Flow Cytometry Analysis: 0.015 µg of this antibody detected Foxp3 in one million C57Bl/6 splenocytes.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable