MAB378

Anti-MAP2 Antibody

CHEMICON^®, mouse monoclonal, AP20

• Synonym(s): Microtubule Associated Protein 2
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41
• Clone: AP20, monoclonal
• Species reactivity: quail, bovine, human, mouse, amphibian, Xenopus, rat, avian
• Application: IHC, WB
• Citations: 116

Properties

• Product Name: Anti-MAP2A Antibody, AP20, ascites fluid, clone AP20, Chemicon^®
• biological source: mouse
• Quality Segment: 100
• antibody form: ascites fluid
• antibody product type: primary antibodies
• clone: AP20, monoclonal
• species reactivity: quail, bovine, human, mouse, amphibian, Xenopus, rat, avian
• manufacturer/tradename: Chemicon^®
• technique(s): immunohistochemistry: suitable, western blot: suitable
• isotype: IgG1
• NCBI accession no.: NM_001039538.1
• UniProt accession no.: P11137
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... MAP2(4133)

Description

General description

MAP-2 (microtubule associated protein-2) is on of several high molecular weight proteins that play an important role in brain microtubule assembly. In addition to its association with microtubules, MAP-2 associates with neurofilaments and actin filaments suggesting that it may guide interaction among microtubules, other cytoskeletal elements, and cytoplasmic organelles (Binder, 1984).

MAP-2 is a stringent marker for neurons. In addition, MAP-2 displays intracellular specificity. In the central nervous system, MAP-2 is confined to neuronal cell bodies and dendrites. There are exceptions, however, where some axons stain positive for small amounts of MAP-2 (Caceres, 1984; Binder, 1986). MAP-2 is uniformly distributed throughout the cell when first expressed in cultured neurons but becomes selectively localized as dendritic development proceeds (Caceres, 1986; Dotti, 1987).

~ 300 kDa

Immunogen

Bovine MAP2

Application

Detect MAP2A using this Anti-MAP2A Antibody, AP20 validated for use in IH & WB.

Immunohistochemistry:
1:50-1:200. AP20 stains dendrites and neuron cells bodies but not neuronal processes and axons. A previous lot of this antibody was used in immunohistochemistry.

Western blot:
SDS-PAGE Profiles: In SDS-PAGE MAP-2 from adult rat migrates as a closely associated doublet having a molecular weight of approximately 300 kD. However, early in brain development (postnatal day 10 in rats), MAP-2 migrates as a single band that is identical to the lower molecular weight band of the adult MAP-2 doublet (MAP-AP20). Later in development (postnatal days 17-18), the mobility of MAP-2 changes to the adult doublet form. (In the spinal cord, conversion to the adult form occurs earlier).

Western blot: A 1:500 dilution was used in a previous lot.

Optimal working dilutions must be determined by end user.

Research Category
Neuroscience

Research Sub Category
Neuronal & Glial Markers

Neurofilament & Neuron Metabolism

Biochem/physiol Actions

MAP2a & b. Does not recognize other variants.

Physical form

Unpurified

Unpurified mouse monoclonal IgG1 ascites fluid in buffer containing 15 mM sodium azide

Preparation Note

Stable for 1 year at -20ºC in undiluted aliquots from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

Analysis Note

Control
Brain tissue

Cultured neurons

Routinely evaluated by Western Blot on PC12 lysates.

Western Blot Analysis:
1:500 dilution of this lot detected MAP 2 on 10 μg of PC12 lysates.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Replaces: MAB3418

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: nwg
• flash_point_f: Not applicable
• flash_point_c: Not applicable