IM37

Anti-MMP-9 (Ab-3) Mouse mAb (56-2A4)

liquid, clone 56-2A4, Calbiochem^®

• Synonym(s): Anti-Gelatinase B, Anti-92 kDa Gelatinase, Anti-Matrix Metalloproteinase 9
• NACRES: NA.41
• UNSPSC Code: 12352203
• Clone: 56-2A4, monoclonal
• Species reactivity: human, rabbit, rat, guinea pig
• Application: —
• Citations: 11

Properties

• biological source: mouse
• Quality Segment: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: 56-2A4, monoclonal
• form: liquid
• contains: ≤0.1% sodium azide as preservative
• species reactivity: human, rabbit, rat, guinea pig
• should not react with: hamster, mouse, bovine
• manufacturer/tradename: Calbiochem^®
• storage condition: OK to freeze, avoid repeated freeze/thaw cycles
• dilution: (Frozen Sections ; Immunoblotting (1 µg/mL); Paraffin Sections )
• isotype: IgG1
• shipped in: wet ice
• storage temp.: −20°C
• target post-translational modification: unmodified
• Gene Information: human ... MMP9(4318)

Description

General description

Purified mouse monoclonal antibody (see application references). Recognizes both the ~92 kDa latent and the ~83 kDa active forms of MMP-9.

Recognizes the ~92 kDa latent and the ~83 kDa active forms of MMP-9 in breast carcinoma tissue.

This Anti-MMP-9 (Ab-3) Mouse mAb (56-2A4) is validated for use in Frozen Sections, Immunoblotting, Paraffin Sections for the detection of MMP-9 (Ab-3).

Immunogen

Human

a synthetic peptide corresponding to amino acids 626-644 of human MMP-9

Application

Frozen Sections (see application references)
Immunoblotting (1 g/ml)
Paraffin Sections (see application references)

Packaging

Please refer to vial label for lot-specific concentration.

Physical form

In 100 mM sodium phosphate buffer, 0.1% BSA, pH 7.0

Preparation Note

Following initial thaw, aliquot and freeze (-20°C).

Analysis Note

Negative Control
MMP-2 protein (Cat. Nos. PF023 or PF037)

Positive Control
MMP-9 protein (Cat. Nos. PF024 or PF038) or breast carcinoma tissue

Other Notes

Cottam, D.W. and Rees, R.C. 1993. Intl. J. Oncol.2, 861.
Stetler-Stevenson, W.G., et al. 1993. FASEB J.7, 1434.
Okada, Y., et al., 1992. J. Biol. Chem.267, 21712.
Woessner, J.F. 1991. FASEB J.5, 2145.
Liotta, L.A. and Stetler-Stevenson, W.G. 1990. in Seminars in Cancer Biology, ed. M.M. Gottesman. Vol. 1, 99.

This antibody does not react with MMP-1, MMP-2, MMP-3 or MMP-13. Antibody should be titrated for optimal results in individual systems.

Legal Information

Manufactured by Daiichi Fine Chemical Co., Ltd. Not available for sale in Japan.

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Toxicity: Standard Handling (A)

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk: WGK 1