CBL582

Anti-CD19 Antibody, clone HD37

clone HD37, Chemicon^®, from mouse

• Synonym(s): B-lymphocyte Surface Antigen B4
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: HD37, monoclonal
• Application: FACS, IHC, IP
• Citations: 7

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified immunoglobulin
• clone: HD37, monoclonal
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• technique(s): flow cytometry: suitable, immunohistochemistry: suitable, immunoprecipitation (IP): suitable
• isotype: IgG1
• NCBI accession no.: NM_001770.4
• UniProt accession no.: P15391
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CD19(930)

Description

Application

Anti-CD19 Antibody, clone HD37 detects level of CD19 & has been published & validated for use in FC, IP, IH.

Identification of CD19 positive B lymphocytes and malignant B cells by indirect immunofluorescence staining

Leukaemia and lymphoma phenotyping

Identification of normal and malignant CD19 positive B lymphocytes in cryostat frozen sections

Immunoprecipitation of the CD19 antigen

Studies of the CD19/21 complex in B cell activation and the control of B cell development

Optimal working dilutions must be determined by the end user.

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

Biochem/physiol Actions

The CD19 antigen (90 kDa) is expressed from the earliest stages of B-progenitor development, on all peripheral B cells including germinal centre B cells and all B cell lines and B cell leukaemias tested. T cell and monocytic cell lines are negative and the antigen is lost on B cell maturations to plasma cells. The antigen is a type I integral membrane glycoprotein whose in vitro inhibition will influence B cell activation and proliferation.

Physical form

Format: Purified

The monoclonal is presented at a concentration of 100μg/1ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.

Preparation Note

For use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.

Other Notes

Replaces: CBL1322

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: WGK 2