CBL188

Anti-Clathrin Antibody, clone CHC5.9

clone CHC5.9, Chemicon^®, from mouse

• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: CHC5.9, monoclonal
• Application: IHC, WB
• Citations: 6

Properties

• biological source: mouse
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: CHC5.9, monoclonal
• species reactivity: bovine, amphibian, rat, pig, human
• manufacturer/tradename: Chemicon^®
• technique(s): immunohistochemistry: suitable, western blot: suitable
• isotype: IgM
• NCBI accession no.: NM_004859.3
• UniProt accession no.: Q00610
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... CLTC(1213)

Description

Immunogen

Coated vesicles (clathrin) of bovine brain.

Application

Anti-Clathrin Antibody, clone CHC5.9 is an antibody against Clathrin for use in WB, IH.

Marker for detection of receptor mediated endocytosis

Suitable for frozen sections

Western blotting KNOW SPECIES

Optimal working dilutions must be determined by the end user.

Research Category
Epitope Tags & General Use

Research Sub Category
Organelle & Cell Markers

Biochem/physiol Actions

This antibody reacts with the clathrin heavy chain from a diverse range of species. In nervous tissue it cross-reacts with another coated vesicle polypeptide of 190 kDa which is not present in isolated clathrin triskelions. In some epithelial cells of certain species the antibody may cross-react with a cytokeratin component. It detects coated vesicles from different organs and tissues (e.g. brain, ovary, mammary gland). : Recognizes bovine, rat and amphibian

Physical form

Format: Purified

The antibody was purified by gel filtration and is presented as 100μg of lyophilysed material. Reconstitute with 1ml distilled water. Final buffer composition is PBS, pH 7.4.

Preparation Note

Maintain at 2-8°C for up to 12 months from date of receipt.

Analysis Note

Control
Positive reactions using HeLa, SV-40 and RVF-SMC cultured cell lines.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 13 - Non Combustible Solids
• wgk: WGK 3