AP101F

Rabbit Anti-Human IgG Antibody, FITC conjugate

Chemicon^®, from rabbit

• UNSPSC Code: 12352203
• NACRES: NA.46
• eCl@ss: 32160702
• Conjugate: FITC conjugate
• Clone: polyclonal
• Application: IF
• Citations: -

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: FITC conjugate
• antibody form: F(ab′)₂ fragment of affinity isolated antibody
• antibody product type: secondary antibodies
• clone: polyclonal
• species reactivity: human
• manufacturer/tradename: Chemicon^®
• technique(s): immunofluorescence: suitable
• shipped in: wet ice
• target post-translational modification: unmodified

Description

Application

DILUTION:

Suggested dilution for most applications: 1:50-1:200

Optimal working dilutions must be determined by the end user.

This Rabbit anti-Human IgG Antibody, FITC conjugate is validated for use in IF for the detection of Human IgG.

Biochem/physiol Actions

Human IgG (H&L)

FLUOROPHORE/PROTEIN:

Approximately 6.9 mg/mg; 2.6 moles FITC per mol IgG

Physical form

Liquid in 0.01 M Phosphate buffer, 0.25 M NaCl pH 7.6 with 15 mg/mL BSA and 0.05% sodium azide.

RECONSTITUTION:

Reconstitute to 1 mg/mL with sterile distilled water.

Preparation Note

Maintain lyophilized product between 2 and 8°C for up to 12 months. After reconstitution the product is stable for several weeks at 2-8°C as an undiluted liquid. For extended storage after reconstitution, add an equal volume of glycerol to make a final concentration of 50% glycerol followed by storage at -20°C in undiluted aliquots for up to 12 months. Please note the concentration of protein (and buffer salts) will decrease to one-half of the original after the addition of glycerol. Avoid repeated freeze/thaw cycles.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Hazard Codes: H412
• Precautionary Statements: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• supp_hazards: EUH032
• Storage Class: 11 - Combustible Solids
• WGK: WGK 3