ABS500
Anti-CDT2 Antibody
serum, from rabbit
Synonym(s):
Denticleless protein homolog, B1- and CUL4-associated factor 2, Lethal(2) denticleless protein homolog, Retinoic acid-regulated nuclear matrix-associated protein
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rabbit
Quality Level
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
mouse, rat, human
technique(s)
western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
dry ice
target post-translational modification
unmodified
Gene Information
human ... DTL(51514)
General description
CDT2, also known as DTL, Denticleless protein homolog, DDB1- and CUL4-associated factor 2, Lethal(2) denticleless protein homolog, or Retinoic acid-regulated nuclear matrix-associated protein, and encoded by the gene DTL/CDT2/CDW1/DCAF2/L2DTL/RAMP, is a component of the DCX(DTL) E3 ubiquitin ligase complex and is the substrate-specific adapter for the DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex required for cell cycle control, DNA damage response and translesion DNA synthesis. The DCX(DTL) complex, also named CRL4(CDT2) complex, mediates the polyubiquitination and subsequent degradation of CDT1 and CDKN1A/p21(CIP1). CDT2 is important because degradation of CDT1 in response to DNA damage is necessary to ensure proper cell cycle regulation of DNA replication. CDT2 is localized to the nucleus primarily but it translocates to the cytoplasm during mitosis. CDT2 is expressed in multiple tissues at varying degrees with high expression in adult placenta, testis, thymus, bone marrow and fetal all fetal tissues, and low expression in adult muscle, spleen and lymph node.
Immunogen
Recombinant protein corresponding to human CDT2.
Application
Anti-CDT2 Antibody detects level of CDT2 & has been published & validated for use in CDT2.
Research Category
Signaling
Signaling
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
Western Blotting Analysis: A representative lot from an independent laboratory detected CDT2 in transfected 293T cell lysates (Abbas, T., et al. (2013). Mol Cell. 49(6):1147-1158.).
Quality
Evaluated by Western Blotting in A431 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CDT2 in 10 µg of A431 cell lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected CDT2 in 10 µg of A431 cell lysate.
Target description
~85 kDa observed
Physical form
Rabbit polyclonal serum containing 0.05% sodium azide.
Unpurified
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class
10 - Combustible liquids
wgk_germany
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Neysha Tirado-Class et al.
Life science alliance, 7(1) (2023-11-09)
DDB1- and CUL4-associated factors (DCAFs) CDT2 and DCAF14 are substrate receptors for Cullin4-RING E3 ubiquitin ligase (CRL4) complexes. CDT2 is responsible for PCNA-coupled proteolysis of substrates CDT1, p21, and SET8 during S-phase of cell cycle. DCAF14 functions at stalled replication
Debra L Evans et al.
Cell cycle (Georgetown, Tex.), 15(1), 95-105 (2016-01-16)
The timely and precise duplication of cellular DNA is essential for maintaining genome integrity and is thus tightly-regulated. During mitosis and G1, the Origin Recognition Complex (ORC) binds to future replication origins, coordinating with multiple factors to load the minichromosome
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