ABS14

Anti-PKA RII Beta Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): protein kinase, cAMP-dependent, regulatory, type II, beta, cAMP-dependent protein kinase type II-beta regulatory chain, cAMP-dependent protein kinase type II-beta regulatory subunit
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: WB
• Citations: 2

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: mouse, human, horse
• species reactivity (predicted by homology): bovine (based on 100% sequence homology), rhesus macaque (based on 100% sequence homology), canine (based on 100% sequence homology), chimpanzee (based on 100% sequence homology), equine (based on 100% sequence homology)
• technique(s): western blot: suitable
• NCBI accession no.: NP_002727
• UniProt accession no.: P31323
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... PRKAR2B(5577)

Description

General description

PKA RII Beta is a type two regulatory chain which mediates membrane association by binding to anchoring proteins, including the MAP2 kinase. When the enzyme is inactive there are two regulatory chains and two catalytic chains. PKA RII Beta is activated by cAMP and produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules.

~ 46 kDa observed

Immunogen

Epitope: cAMP2 domain

KLH-conjugated linear peptide corresponding to human PKA RII Beta at the cAMP2 domain.

Application

Anti-PKA RII Beta Antibody is an antibody against PKA RII Beta for use in WB.

Research Category
Signaling

Research Sub Category
Kinases & Phosphatases

Western Blot Analysis: 0.5 µg/mL from a previous lot detected PKA RII Beta on 10 µg of mouse brain tissue lysate.

Biochem/physiol Actions

This antibody recognizes PKA RII Beta at the cAMP2 domain.

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
Human brain tissue lysate

Evaluated by Western Blot in human brain tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected PKA RII Beta on 10 µg of human brain tissue lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable