ABE1845

Anti-GABPA Antibody

from rabbit

• Synonym(s): GA-binding protein alpha chain, GABP subunit alpha, GABPA
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: purified antibody
• antibody product type: primary antibodies
• clone: polyclonal
• species reactivity: human, rat, mouse
• species reactivity (predicted by homology): monkey (based on 100% sequence homology), bovine (based on 100% sequence homology), canine (based on 100% sequence homology)
• technique(s): ChIP: suitable; western blot: suitable
• NCBI accession no.: NP_032091
• UniProt accession no.: Q00422
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... GABPA(2551) ; mouse ... Gabpa(14390)

Description

General description

GA-binding protein alpha chain (UniProt Q00422; also known as GA repeat binding protein alpha, GABP subunit alpha, GABPalpha, NRF-2, Nuclear respiratory factor-2) is encoded by the Gabpa gene (also known as E4tf1a) in murine species (Gene ID 14390). GABPalpha is a redox-responsive transcription factor that plays an anti-oxidant, pro-survival role in mitochondrial biogenesis process. GABPalpha is upregulated in response to oxidant exposure or increased cellular reactive oxygen species (ROS), where it mediates the transcription of mitochondrial transcription factor A (mtTFA) that is needed for mitochondrial DNA transcription.

Immunogen

Epitope: Near C-terminus

KLH-conjugated linear peptide corresponding to mouse GABPA near the C-terminus.

Application

Detect GABPA using this Anti-GABPA Antibody validated for use in Western Blotting and Chromatin Immunoprecipitation (ChIP).

Research Category
Epigenetics & Nuclear Function

Research Sub Category
Nuclear Receptors

Western Blotting Analysis: A representative lot detected GABPA in mouse liver nuclear proteins (Cherry, A.D., et al. (2014). J. Biol. Chem. 289(1):41-52).
Chromatin Immunoprecipitation Analysis: A representative lot detected GABPA occupancy at the Sod2 promoter region by ChIP using liver chromatin preparation from mice after S. aureus inoculation (Cherry, A.D., et al. (2014). J. Biol. Chem. 289(1):41-52).

Quality

Evaluated by Western Blotting in mouse liver tissue lysate.

Western Blotting Analysis: 2.0 µg/mL of this antibody detected GABPA in 10 µg of mouse liver tissue lysate.

Target description

~55 kDa observed

Physical form

Format: Purified

Protein A purified

Purified rabbit polyclonal antibody in buffer containing PBS and 0.01% Sodium Azide.

Storage and Stability

Stable for 1 year at 2-8°C from date of receipt.

Other Notes

Concentration: Please refer to lot specific datasheet.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 10 - Combustible liquids
• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable