AB5589

Anti-Potassium Channel Kv1.3 Antibody, extracellular domain

Chemicon^®, from rabbit

• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: FACS, WB
• Citations: 3

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: rat, human
• manufacturer/tradename: Chemicon^®
• technique(s): flow cytometry: suitable, western blot: suitable
• NCBI accession no.: NM_002232.3
• UniProt accession no.: P22001
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... KCNA3(3738)

Description

Immunogen

Epitope: extracellular domain

Highly purified peptide corresponding to residues 263-275 of human Kv1.3 (Accession P22001).

Application

Research Category
Neuroscience

Research Sub Category
Ion Channels & Transporters

This Anti-Potassium Channel Kv1.3 Antibody, extracellular domain is validated for use in FC, WB for the detection of Potassium Channel Kv1.3.

Western blotting: 1:200-1:500 using ECL on rat brain membranes.

Indirect flow cytometry: 0.5-5 μg of antibody per 1x10e6cells.

Dilutions should be made using a carrier protein such as BSA (1-3%).

Optimal working dilutions must be determined by the end user.

Biochem/physiol Actions

Recognizes Kv1.3, extracellular (Kcna3).

Physical form

Affinity purified immunoglobulin. Lyophilized from PBS, pH 7.4, containing 1% BSA and 0.05% sodium azide. Reconstitute with 200 μL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).

Preparation Note

Maintain lyophilized material at -20°C for up to 6 months after date of receipt. After reconstitution maintain antibody and control peptide at -20°C in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.

Analysis Note

Control
Included free of charge with the antibody is 40 μg of control antigen (lyophilized powder). For negative control, preincubate 5 μg of purified peptide with 1 μg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• hcodes: H412
• pcodes: P273 - P501
• Hazard Classifications: Aquatic Chronic 3
• Storage Class: 11 - Combustible Solids
• wgk: WGK 3