AB2953

Anti-ATG3 Antibody

from rabbit, purified by affinity chromatography

• Synonym(s): ATG3 autophagy related 3 homolog (S. cerevisiae), APG3 autophagy 3-like (S. cerevisiae), autophagy-related protein 3, autophagy Apg3p/Aut1p-like, APG3 autophagy 3-like, Protein PC3-96
• UNSPSC Code: 12352203
• NACRES: NA.41
• eCl@ss: 32160702
• Conjugate: unconjugated
• Clone: polyclonal
• Application: WB
• Citations: -

Properties

• biological source: rabbit
• Quality Segment: 100
• conjugate: unconjugated
• antibody form: affinity isolated antibody
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• technique(s): western blot: suitable
• NCBI accession no.: NP_071933
• UniProt accession no.: Q9NT62
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... ATG3(64422)

Description

General description

ATG3 is thought to be an autophagy-related ubiquitin carrier protein (E2)-like enzyme that is the go between for the conjugation on ATG8 and phosphatidylethanolamine (PE). This compound is necessary for autophagy, which is a process in which a cell is degraded by its own lysosomal process.

~37 kDa observed

Immunogen

GST-tagged recombinant protein corresponding to human ATG3.

Application

Anti-ATG3 Antibody detects level of ATG3 & has been published & validated for use in WB.

Research Category
Apoptosis & Cancer

Research Sub Category
Apoptosis - Additional

Biochem/physiol Actions

Other homologies: Ape, rhesus macaque, equine, porcine, and bovine (99% sequence homology). Mouse (98% sequence homology). Rat (97% sequence homology).

Physical form

Affinity purified

Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Preparation Note

Stable for 1 year at 2-8°C from date of receipt.

Analysis Note

Control
HL-60 cell lysate

Evaluated by Western Blot in HL-60 cell lysate.

Western Blot Analysis: 0.1 µg/mL of this antibody detected ATG3 in 10 µg of HL-60 cell lysate.

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable