After verification, an anti-doublecortin antibody recommended for free-floating application on mouse brain tissue is not currently available. However, a citation on AB2253 for free-floating application on mouse brain tissue was found and may be of interest for reference.
Product: AB2253 Sigma-Aldrich Anti-Doublecortin Antibody
Publication: Nat Commun. 2015 Mar 26;6:6606
Title: Aberrant hippocampal neurogenesis contributes to epilepsy and associated cognitive decline
PMID: 25808087
AB2253
Anti-Doublecortin Antibody
serum, from guinea pig
Anti-Doublecortin Antibody
Synonym(s):
Anti-Doublecortin Antibody, AB2253 | Sigma-Aldrich
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| Size/SKU | Availability | Price |
|---|---|---|
50 μL | Check Cart for Availability | $661.00 |
About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
polyclonal
Application:
IF, IHC, WB
Citations:
234
biological source
guinea pig
Quality Segment
conjugate
unconjugated
antibody form
serum
antibody product type
primary antibodies
clone
polyclonal
species reactivity
rat, mouse
species reactivity (predicted by homology)
human (based on 100% sequence homology)
technique(s)
immunofluorescence: suitable (Frozen tissue sections), immunohistochemistry: suitable (Paraffin), western blot: suitable
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... DCDC1(341019)
General description
Anti-Doublecortin, Cat. No. AB2253, is a guinea pig polyclonal antibody that detects Doublecortin and is tested for use in Immunohistochemistry (Paraffin) and Immunofluorescence, and Western Blotting.
Target molecular weight ~42 kDa observed;40.61 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
~45 kDa observed; 40.57 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
Immunogen
Epitope: C-Terminus
Recombinant Peptide.
Application
Anti-Doublecortin Antibody has been used in immunohistochemistry and immunofluorescence staining.
Biochem/physiol Actions
Doublecortin (DCX) is identified as an important marker for neurogenesis. It serves as a valuable tool for studying and comparing the process of neurogenesis across different species.[1]
Reactivity with other species has not been determined.
This Guinea pig polyclonal antibody detects doublecortin in murine and rat species. It targets an epitope with in 17 amino acids from the C-terminal region.
Physical form
GST Depletion
GST-Depleted Guinea Pig Sera with 0.05% Sodium Azide
Preparation Note
Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Control
Rat Brain lysate.
Rat Brain lysate.
Evaluated by Western Blotting in rat brain tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Doublecortin in rat brain tissue lysate.
Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Doublecortin in rat brain tissue lysate.
Other Notes
Replaces: AB5910
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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This Item | |||
|---|---|---|---|
| Quality Level 100 | Quality Level 200 | Quality Level 200 | Quality Level - |
| conjugate unconjugated | conjugate unconjugated | conjugate unconjugated | conjugate unconjugated |
| biological source guinea pig | biological source rabbit | biological source rabbit | biological source rabbit |
| antibody form serum | antibody form affinity isolated antibody | antibody form affinity isolated antibody | antibody form affinity isolated antibody |
| technique(s) immunofluorescence: suitable (Frozen tissue sections), western blot: suitable, immunohistochemistry: suitable (Paraffin) | technique(s) western blot: 0.5-1 μg/mL using HEK-293T cells expressing human doublecortin and rat brain extract (S1 fraction), western blot: 1-2 μg/mL using mouse brain extract (S1 fraction) | technique(s) western blot: 1-2 μg/mL using HEK-293T cells expressing human doublecortin. Also, mouse and rat brain extract (S1 fraction). | technique(s) ELISA: 1:10000, western blot: 1:500-1:1000 |
| UniProt accession no. | UniProt accession no. | UniProt accession no. | UniProt accession no. |
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
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Is it possible to obtain a small aliquot of specific antibodies for testing purposes to determine their suitability for staining Doublecortin (DCX) in immunohistochemistry on free-floating mouse brain tissue fixed with PFA? The antibodies of interest are the guinea pig polyclonal and the goat polyclonal.
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