69839-M

T4 DNA Ligase - Novagen

Qualified under the most stringent cloning conditions

• Synonym(s): DNA Ligation
• UNSPSC Code: 41106310
• NACRES: NA.54

Properties

• Quality Level: 200
• manufacturer/tradename: Novagen^®
• storage condition: OK to freeze; avoid repeated freeze/thaw cycles
• shipped in: dry ice; wet ice
• storage temp.: −20°C

Description

General description

T4 DNA Ligase catalyzes the formation of phosphodiester bonds between 3′-hydroxyl and 5′-phosphate groups of adjacent DNA and RNA nucleotides in blunt-end or cohesive-end configurations. The enzyme joins RNA and DNA strands only in duplex molecules and does not join single-stranded nucleic acids. Novagen T4 DNA Ligase is rigorously tested in a blue-white cloning assay under conditions that maximize exposure of DNA ends to any contaminating nucleases. The enzyme is qualified for any ligation or cloning application.

Warning

Toxicity: Standard Handling

Unit Definition

4 Weiss units/µl; one Weiss unit is defined as the amount of enzyme required to catalyze the exchange of 1 nmole of ³²P from pyrophosphate into ATP as Norit^®-absorbable material in 20 minutes at 37°C. 0.01 Weiss unit is the amount of enzyme required to ligate > 95% of 1 µg bacteriophage λ Hind III fragments at 16°C in 20 minutes.

Legal Information

NOVAGEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Norit is a registered trademark of Norit N.V.

Safety Information

• wgk_germany: WGK 2
• flash_point_f: Not applicable
• flash_point_c: Not applicable